Acridine orange particles in cultured fibroblasts

A comparative study of macular corneal dystrophy, systemic mucopolysaccharidoses types I-H and II, and normal controls

G. K. Klintworth, H. K. Hawkins, C. F. Smith

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Vital staining with the fluorescent dye, acridine orange, was evaluated as a means of detecting abnormalities of lysosomes in cultivated fibroblasts of patients with macular corneal dystrophy and mucopolysaccharidoses types I-H (Hurler's syndrome) and type II (Hunter's syndrome). Multiple cultures were compared with normal fibroblasts using a 'double-masked' design to exclude observer bias. Cells of patients with the mucopolysaccharidoses were easily and accurately separated from other fibroblasts. Contrary to a recent report, corneal fibroblasts of patients with macular corneal dystrophy were indistinguishable from control cells.

Original languageEnglish (US)
Pages (from-to)297-299
Number of pages3
JournalArchives of Pathology and Laboratory Medicine
Volume103
Issue number6
StatePublished - 1979
Externally publishedYes

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Mucopolysaccharidosis II
Mucopolysaccharidosis I
Acridine Orange
Fibroblasts
Mucopolysaccharidoses
Observer Variation
Lysosomes
Fluorescent Dyes
Staining and Labeling
Corneal type 1 Macular dystrophy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

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