Vital staining with the fluorescent dye, acridine orange, was evaluated as a means of detecting abnormalities of lysosomes in cultivated fibroblasts of patients with macular corneal dystrophy and mucopolysaccharidoses types I-H (Hurler's syndrome) and type II (Hunter's syndrome). Multiple cultures were compared with normal fibroblasts using a 'double-masked' design to exclude observer bias. Cells of patients with the mucopolysaccharidoses were easily and accurately separated from other fibroblasts. Contrary to a recent report, corneal fibroblasts of patients with macular corneal dystrophy were indistinguishable from control cells.
|Original language||English (US)|
|Number of pages||3|
|Journal||Archives of Pathology and Laboratory Medicine|
|State||Published - Dec 1 1979|
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Medical Laboratory Technology