Acrylonitrile interaction with testicular DNA in rats

Ahmed E. Ahmed, Sherif Z. Abdel‐Rahman, Amr M.‐Al Nour Deen

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

In the present study we report the in vivo interaction of acrylonitrile (VCN) with testicular tissue in rats. Covalent binding of radioactivity to testicular tissue DNA was examined for a period of 72 hr after a single oral dose (46.5 mg/kg) of [2, 3‐14C] VCN. Maximal covalent binding was observed at 0.5 hr (8.9 μmol VCN equivalent/mol nucleotide). Binding decreased gradually thereafter but was still detected (2.5 μmol VCN equivalent/mol nucleotide) at 72 hr following VCN administration. Further, we examined the effects of VCN on DNA synthesis and repair in the testes of rats following a single oral dose (46.5 mg/kg) of VCN to clarify the impact of the covalent binding observed on the testicular genetic material. A significant decrease in DNA synthesis (80% of control) was observed at 0.5 hr after treatment. At 24 hr following acrylonitrile administration, testicular DNA synthesis was severely inhibited (38% of control). Testicular DNA repair was increased 1.5‐fold at 0.5 hr and more than 3.3‐fold at 24 hr following treatment with VCN. These results suggest that VCN can act as a multipotent genotoxic agent by alkylating DNA in testicular tissue and may affect the male reproductive function by interfering with testicular DNA synthesis and repair processes.

Original languageEnglish (US)
Pages (from-to)5-11
Number of pages7
JournalJournal of biochemical toxicology
Volume7
Issue number1
DOIs
StatePublished - Jan 1 1992

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Keywords

  • Acrylonitrile
  • Covalent Binding
  • DNA Repair
  • DNA Synthesis
  • Testicular Effect

ASJC Scopus subject areas

  • Toxicology

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