Acrylonitrile interaction with testicular DNA in rats.

A. E. Ahmed, Sherif Abdel-Rahman, A. M. Nour-al Deen

Research output: Contribution to journalArticle

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Abstract

In the present study we report the in vivo interaction of acrylonitrile (VCN) with testicular tissue in rats. Covalent binding of radioactivity to testicular tissue DNA was examined for a period of 72 hr after a single oral dose (46.5 mg/kg) of [2,3-14C] VCN. Maximal covalent binding was observed at 0.5 hr (8.9 mumol VCN equivalent/mol nucleotide). Binding decreased gradually thereafter but was still detected (2.5 mumol VCN equivalent/mol nucleotide) at 72 hr following VCN administration. Further, we examined the effects of VCN on DNA synthesis and repair in the testes of rats following a single oral dose (46.5 mg/kg) of VCN to clarify the impact of the covalent binding observed on the testicular genetic material. A significant decrease in DNA synthesis (80% of control) was observed at 0.5 hr after treatment. At 24 hr following acrylonitrile administration, testicular DNA synthesis was severely inhibited (38% of control). Testicular DNA repair was increased 1.5-fold at 0.5 hr and more than 3.3-fold at 24 hr following treatment with VCN. These results suggest that VCN can act as a multipotent genotoxic agent by alkylating DNA in testicular tissue and may affect the male reproductive function by interfering with testicular DNA synthesis and repair processes.

Original languageEnglish (US)
Pages (from-to)5-11
Number of pages7
JournalJournal of Biochemical Toxicology
Volume7
Issue number1
StatePublished - Mar 1992

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Acrylonitrile
Rats
DNA Repair
DNA
Nucleotides
Repair
Tissue
Alkylating Agents
Radioactivity
Testis
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Acrylonitrile interaction with testicular DNA in rats. / Ahmed, A. E.; Abdel-Rahman, Sherif; Nour-al Deen, A. M.

In: Journal of Biochemical Toxicology, Vol. 7, No. 1, 03.1992, p. 5-11.

Research output: Contribution to journalArticle

Ahmed, A. E. ; Abdel-Rahman, Sherif ; Nour-al Deen, A. M. / Acrylonitrile interaction with testicular DNA in rats. In: Journal of Biochemical Toxicology. 1992 ; Vol. 7, No. 1. pp. 5-11.
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