Abstract
Human milk neutrophils and macrophages were examined by flow cytometry to determine whether they displayed phenotypic markers of activation. The markers were CD11b and L-selectin, which are increased or shed, respectively, from the surface of activated neutrophils. Phenotypic features of milk neutrophils and macrophages were similar to blood neutrophils stimulated with fMLP: plasma membrane expression of CD11b was increased and L-selectin was decreased. After blood neutrophils were incubated in acellular milk, their expression of CD11b increased and L-selectin decreased. The activation was not affected by trypsin but was significantly decreased by treating acellular milk with chloroform or ether. Sedimentation studies suggested that particulate fractions of milk were active. Further, the activation was partly blocked by treating target blood neutrophils with cytochalasin B. Thus, human milk neutrophils are activated, and the activation may be due partly to phagocytosis of membranous structures in milk.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 97-104 |
| Number of pages | 8 |
| Journal | Journal of Leukocyte Biology |
| Volume | 54 |
| Issue number | 2 |
| State | Published - 1993 |
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Keywords
- CD11b
- human milk
- L-selectin
- neutrophils
ASJC Scopus subject areas
- Cell Biology
Cite this
Activated neutrophils and neutrophil activators in human milk : Increased expression of CD11b and decreased expression of L-selectin. / Keeney, S. E.; Schmalstieg, F. C.; Palkowetz, K. H.; Rudloff, H. E.; Le, B. M.; Goldman, A. S.
In: Journal of Leukocyte Biology, Vol. 54, No. 2, 1993, p. 97-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activated neutrophils and neutrophil activators in human milk
T2 - Increased expression of CD11b and decreased expression of L-selectin
AU - Keeney, S. E.
AU - Schmalstieg, F. C.
AU - Palkowetz, K. H.
AU - Rudloff, H. E.
AU - Le, B. M.
AU - Goldman, A. S.
PY - 1993
Y1 - 1993
N2 - Human milk neutrophils and macrophages were examined by flow cytometry to determine whether they displayed phenotypic markers of activation. The markers were CD11b and L-selectin, which are increased or shed, respectively, from the surface of activated neutrophils. Phenotypic features of milk neutrophils and macrophages were similar to blood neutrophils stimulated with fMLP: plasma membrane expression of CD11b was increased and L-selectin was decreased. After blood neutrophils were incubated in acellular milk, their expression of CD11b increased and L-selectin decreased. The activation was not affected by trypsin but was significantly decreased by treating acellular milk with chloroform or ether. Sedimentation studies suggested that particulate fractions of milk were active. Further, the activation was partly blocked by treating target blood neutrophils with cytochalasin B. Thus, human milk neutrophils are activated, and the activation may be due partly to phagocytosis of membranous structures in milk.
AB - Human milk neutrophils and macrophages were examined by flow cytometry to determine whether they displayed phenotypic markers of activation. The markers were CD11b and L-selectin, which are increased or shed, respectively, from the surface of activated neutrophils. Phenotypic features of milk neutrophils and macrophages were similar to blood neutrophils stimulated with fMLP: plasma membrane expression of CD11b was increased and L-selectin was decreased. After blood neutrophils were incubated in acellular milk, their expression of CD11b increased and L-selectin decreased. The activation was not affected by trypsin but was significantly decreased by treating acellular milk with chloroform or ether. Sedimentation studies suggested that particulate fractions of milk were active. Further, the activation was partly blocked by treating target blood neutrophils with cytochalasin B. Thus, human milk neutrophils are activated, and the activation may be due partly to phagocytosis of membranous structures in milk.
KW - CD11b
KW - human milk
KW - L-selectin
KW - neutrophils
UR - http://www.scopus.com/inward/record.url?scp=0027284142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027284142&partnerID=8YFLogxK
M3 - Article
VL - 54
SP - 97
EP - 104
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 2
ER -