Activated neutrophils and neutrophil activators in human milk

Increased expression of CD11b and decreased expression of L-selectin

S. E. Keeney, F. C. Schmalstieg, K. H. Palkowetz, H. E. Rudloff, B. M. Le, A. S. Goldman

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Human milk neutrophils and macrophages were examined by flow cytometry to determine whether they displayed phenotypic markers of activation. The markers were CD11b and L-selectin, which are increased or shed, respectively, from the surface of activated neutrophils. Phenotypic features of milk neutrophils and macrophages were similar to blood neutrophils stimulated with fMLP: plasma membrane expression of CD11b was increased and L-selectin was decreased. After blood neutrophils were incubated in acellular milk, their expression of CD11b increased and L-selectin decreased. The activation was not affected by trypsin but was significantly decreased by treating acellular milk with chloroform or ether. Sedimentation studies suggested that particulate fractions of milk were active. Further, the activation was partly blocked by treating target blood neutrophils with cytochalasin B. Thus, human milk neutrophils are activated, and the activation may be due partly to phagocytosis of membranous structures in milk.

Original languageEnglish (US)
Pages (from-to)97-104
Number of pages8
JournalJournal of Leukocyte Biology
Volume54
Issue number2
StatePublished - 1993

Fingerprint

L-Selectin
Human Milk
Neutrophils
Milk
Macrophages
Neutrophil Activation
Cytochalasin B
Chloroform
Phagocytosis
Ether
Trypsin
Flow Cytometry
Cell Membrane

Keywords

  • CD11b
  • human milk
  • L-selectin
  • neutrophils

ASJC Scopus subject areas

  • Cell Biology

Cite this

Keeney, S. E., Schmalstieg, F. C., Palkowetz, K. H., Rudloff, H. E., Le, B. M., & Goldman, A. S. (1993). Activated neutrophils and neutrophil activators in human milk: Increased expression of CD11b and decreased expression of L-selectin. Journal of Leukocyte Biology, 54(2), 97-104.

Activated neutrophils and neutrophil activators in human milk : Increased expression of CD11b and decreased expression of L-selectin. / Keeney, S. E.; Schmalstieg, F. C.; Palkowetz, K. H.; Rudloff, H. E.; Le, B. M.; Goldman, A. S.

In: Journal of Leukocyte Biology, Vol. 54, No. 2, 1993, p. 97-104.

Research output: Contribution to journalArticle

Keeney, SE, Schmalstieg, FC, Palkowetz, KH, Rudloff, HE, Le, BM & Goldman, AS 1993, 'Activated neutrophils and neutrophil activators in human milk: Increased expression of CD11b and decreased expression of L-selectin', Journal of Leukocyte Biology, vol. 54, no. 2, pp. 97-104.
Keeney, S. E. ; Schmalstieg, F. C. ; Palkowetz, K. H. ; Rudloff, H. E. ; Le, B. M. ; Goldman, A. S. / Activated neutrophils and neutrophil activators in human milk : Increased expression of CD11b and decreased expression of L-selectin. In: Journal of Leukocyte Biology. 1993 ; Vol. 54, No. 2. pp. 97-104.
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