Abstract
The effect of formyl-Met-Leu-Phe- (fMLP-) activated feline neutrophil granulocytes on endothelium-dependent and independent relaxations was studied in the middle cerebral artery of the cat in vitro. Endothelium-dependent relaxations caused by acetylcholine and ATP were markedly inhibited after 30 minutes of incubation of the vessels with neutrophils (5000 cells/μl) in the presence of 5 μM fMLP, followed by a replacement of the bath solution in order to remove the neutrophils from the medium. Direct vasorelaxations in response to the nitric oxide donor compound SIN-1, however, remained unchanged. Both neutrophils and fMLP caused transient contractions during the incubation period. The present study provides direct evidence for the ability of activated neutrophils to cause an inhibition of vascular endothelium-dependent responses in vitro.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1087-1094 |
| Number of pages | 8 |
| Journal | Life Sciences |
| Volume | 49 |
| Issue number | 15 |
| DOIs | |
| State | Published - 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pharmacology
Cite this
Activated neutrophils inhibit cerebrovascular endothelium-dependent relaxations in vitro. / Csaki, Csilla; Szabo, Csaba; Benyo, Zoltan; Reivich, Martin; Kovach, Arisztid G B.
In: Life Sciences, Vol. 49, No. 15, 1991, p. 1087-1094.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activated neutrophils inhibit cerebrovascular endothelium-dependent relaxations in vitro
AU - Csaki, Csilla
AU - Szabo, Csaba
AU - Benyo, Zoltan
AU - Reivich, Martin
AU - Kovach, Arisztid G B
PY - 1991
Y1 - 1991
N2 - The effect of formyl-Met-Leu-Phe- (fMLP-) activated feline neutrophil granulocytes on endothelium-dependent and independent relaxations was studied in the middle cerebral artery of the cat in vitro. Endothelium-dependent relaxations caused by acetylcholine and ATP were markedly inhibited after 30 minutes of incubation of the vessels with neutrophils (5000 cells/μl) in the presence of 5 μM fMLP, followed by a replacement of the bath solution in order to remove the neutrophils from the medium. Direct vasorelaxations in response to the nitric oxide donor compound SIN-1, however, remained unchanged. Both neutrophils and fMLP caused transient contractions during the incubation period. The present study provides direct evidence for the ability of activated neutrophils to cause an inhibition of vascular endothelium-dependent responses in vitro.
AB - The effect of formyl-Met-Leu-Phe- (fMLP-) activated feline neutrophil granulocytes on endothelium-dependent and independent relaxations was studied in the middle cerebral artery of the cat in vitro. Endothelium-dependent relaxations caused by acetylcholine and ATP were markedly inhibited after 30 minutes of incubation of the vessels with neutrophils (5000 cells/μl) in the presence of 5 μM fMLP, followed by a replacement of the bath solution in order to remove the neutrophils from the medium. Direct vasorelaxations in response to the nitric oxide donor compound SIN-1, however, remained unchanged. Both neutrophils and fMLP caused transient contractions during the incubation period. The present study provides direct evidence for the ability of activated neutrophils to cause an inhibition of vascular endothelium-dependent responses in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0025895881&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025895881&partnerID=8YFLogxK
U2 - 10.1016/0024-3205(91)90596-4
DO - 10.1016/0024-3205(91)90596-4
M3 - Article
C2 - 1895871
AN - SCOPUS:0025895881
VL - 49
SP - 1087
EP - 1094
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 15
ER -