Activation and impairment of platelet function in vitro by fatty acid ethyl ester, a nonoxidative ethanol metabolite: Effect of fatty acid ethyl esters on human platelets

Raneem O. Salem, Michael Laposata

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Background: The goal of this study was to investigate the effect of fatty acid ethyl esters (FAEE), nonoxidative metabolites of ethanol, on platelet function. We hypothesized that FAEE increase the risk of bleeding by producing an alteration in platelet membrane structure or function. Methods: Isolated human platelets incubated with FAEE were prepared and multiple assays for platelet activation were performed; β-thromboglobulin release from platelet granules, platelet aggregation, arachidonate release from phospholipids, and intracellular cyclic AMP (cAMP) levels. We examined also the combined effect of epinephrine and FAEE on platelet aggregation. Results: FAEE induced platelet shape change, release of α granules and release of arachidonate from phospholipids without an increase in eicosanoid production and decreased cAMP levels. The platelets did not aggregate in response to FAEE alone, but did shorten the time to maximum aggregation with epinephrine. Conclusion: These studies show that FAEE potentiate platelet activation but do not induce aggregation, presumably because they do not stimulate thromboxane A2 production.

Original languageEnglish (US)
Pages (from-to)2079-2088
Number of pages10
JournalAlcoholism: Clinical and Experimental Research
Volume30
Issue number12
DOIs
StatePublished - Dec 1 2006

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Keywords

  • Alcohol
  • Arachidonic Acid Release
  • Ethanol
  • Fatty Acid Ethyl Esters
  • Lipid
  • Platelet Aggregation
  • cAMP

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

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