Activation of aldose reductase by nonenzymatic glycosylation.

Satish Srivastava, Naseem Ansari, A. Bhatnagar, G. Hair, S. Liu, B. Das

Research output: Contribution to journalArticle

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Abstract

Incubation of human erythrocyte with 50 mM glucose results in glycosylation of aldose reductase besides hemoglobin A and other proteins. The glycosylation of aldose reductase is established by adsorption of the enzyme on phenyl boronate (PBA-60) column. Furthermore, the enzyme was purified to an apparent homogeneity from the erythrocytes incubated with 50 mM glucose and the glycosylation was quantitated by reduction with tritiated sodium borohydride. The glycosylated aldose reductase exhibits lower Km for glucose and NADPH as compared to unglycosylated enzyme and is not inhibited by phosphorylated intermediates such as ADP, 1,3-DPG, 2,3-DPG and 3-PGA, whereas physiological concentrations of these intermediates almost completely inhibit the unglycosylated enzyme. In addition, the glycosylated enzyme is less susceptible to aldose reductase inhibitors such as sorbinil and alrestatin. In hyperglycemia, blood glucose higher than 11 mM, almost all the aldose reductase is glycosylated.

Original languageEnglish (US)
Pages (from-to)171-184
Number of pages14
JournalProgress in Clinical and Biological Research
Volume304
StatePublished - 1989

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Aldehyde Reductase
Glycosylation
Enzymes
Glucose
Erythrocytes
Prostaglandins A
Hemoglobin A
2,3-Diphosphoglycerate
NADP
Hyperglycemia
Adenosine Diphosphate
Adsorption
Blood Glucose
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Srivastava, S., Ansari, N., Bhatnagar, A., Hair, G., Liu, S., & Das, B. (1989). Activation of aldose reductase by nonenzymatic glycosylation. Progress in Clinical and Biological Research, 304, 171-184.

Activation of aldose reductase by nonenzymatic glycosylation. / Srivastava, Satish; Ansari, Naseem; Bhatnagar, A.; Hair, G.; Liu, S.; Das, B.

In: Progress in Clinical and Biological Research, Vol. 304, 1989, p. 171-184.

Research output: Contribution to journalArticle

Srivastava, S, Ansari, N, Bhatnagar, A, Hair, G, Liu, S & Das, B 1989, 'Activation of aldose reductase by nonenzymatic glycosylation.', Progress in Clinical and Biological Research, vol. 304, pp. 171-184.
Srivastava, Satish ; Ansari, Naseem ; Bhatnagar, A. ; Hair, G. ; Liu, S. ; Das, B. / Activation of aldose reductase by nonenzymatic glycosylation. In: Progress in Clinical and Biological Research. 1989 ; Vol. 304. pp. 171-184.
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AU - Liu, S.

AU - Das, B.

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