Activation of hepatic proliferation-associated transcription factors by lipopolysaccharide

R. D. Beauchamp, John Papaconstantinou, A. M. Henderson, H. M. Sheng, Courtney Townsend, J. C. Thompson, N. V. Christou, R. A. Barke, T. Billiar, R. A. Forse, C. C. Baker

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background. The hepatic acute-phase response is the result of reprogramming of gene expression in the liver. Similar acute-phase responses occur in regenerating liver after partial hepatectomy and are preceded by increases in the expression of a set of transcriptional regulatory proteins that are encoded by 'immediate-early' genes. The purpose of this study was to determine whether acute systemic inflammation after lipopolysaccharide injection induces hepatic immediate-early genes that are induced by partial hepatectomy. Methods. Two- to 4-month-old Balb/c mice received intraperitoneal Escherichia coli lipopolysaccharide (0111:B4; 100 μg), and total liver RNA, nuclear protein extracts, or total liver protein lysates were obtained at 0, 1, 3, 12, and 24 hours. RNA blot hybridization analysis was used to determine steady-state messenger RNA levels for c-jun, jun-B, jun-D, c-fos, fos-B, fra-1, nup475, and zif268. Specific nuclear protein- binding activity was determined by gel mobility shift assay. The protein c- Jun was detected by antibody-blocking experiments, and Jun-B was detected by gel supershift assay of the activating protein (AP-1) complex. Steady-state Jun-B levels were determined by immunoblot analysis. Results. Intraperitoneal injection of lipopolysaccharide is followed by induction (from fivefold to 13-fold) of c-jun, jun-B, c-fos, zif268, and nup475 messenger RNAs in the liver. Lipopolysaccharide induced increases in AP-1 and Zif268 consensus DNA- binding activity in mouse liver. The proteins c-Jun and Jun-B are detected in the AP-1 complex after administration of lipopolysaccharide. Conclusions. The induction of hepatic immediate-early genes after lipopolysaccharide is similar to that that follows partial hepatectomy. These transcription factors likely have important roles in the reprogramming of gene expression that leads to the acute-phase response.

Original languageEnglish
Pages (from-to)367-377
Number of pages11
JournalSurgery
Volume116
Issue number2
StatePublished - 1994

Fingerprint

Lipopolysaccharides
Transcription Factors
Liver
Acute-Phase Reaction
Immediate-Early Genes
Transcription Factor AP-1
Hepatectomy
Proto-Oncogene Proteins c-jun
Nuclear Proteins
Gels
RNA
Gene Expression
Dilatation and Curettage
Messenger RNA
Proteins
Blocking Antibodies
Electrophoretic Mobility Shift Assay
Intraperitoneal Injections
Protein Binding
Escherichia coli

ASJC Scopus subject areas

  • Surgery

Cite this

Beauchamp, R. D., Papaconstantinou, J., Henderson, A. M., Sheng, H. M., Townsend, C., Thompson, J. C., ... Baker, C. C. (1994). Activation of hepatic proliferation-associated transcription factors by lipopolysaccharide. Surgery, 116(2), 367-377.

Activation of hepatic proliferation-associated transcription factors by lipopolysaccharide. / Beauchamp, R. D.; Papaconstantinou, John; Henderson, A. M.; Sheng, H. M.; Townsend, Courtney; Thompson, J. C.; Christou, N. V.; Barke, R. A.; Billiar, T.; Forse, R. A.; Baker, C. C.

In: Surgery, Vol. 116, No. 2, 1994, p. 367-377.

Research output: Contribution to journalArticle

Beauchamp, RD, Papaconstantinou, J, Henderson, AM, Sheng, HM, Townsend, C, Thompson, JC, Christou, NV, Barke, RA, Billiar, T, Forse, RA & Baker, CC 1994, 'Activation of hepatic proliferation-associated transcription factors by lipopolysaccharide', Surgery, vol. 116, no. 2, pp. 367-377.
Beauchamp, R. D. ; Papaconstantinou, John ; Henderson, A. M. ; Sheng, H. M. ; Townsend, Courtney ; Thompson, J. C. ; Christou, N. V. ; Barke, R. A. ; Billiar, T. ; Forse, R. A. ; Baker, C. C. / Activation of hepatic proliferation-associated transcription factors by lipopolysaccharide. In: Surgery. 1994 ; Vol. 116, No. 2. pp. 367-377.
@article{a55ca63260dd4f0da9fea079fc73c1a8,
title = "Activation of hepatic proliferation-associated transcription factors by lipopolysaccharide",
abstract = "Background. The hepatic acute-phase response is the result of reprogramming of gene expression in the liver. Similar acute-phase responses occur in regenerating liver after partial hepatectomy and are preceded by increases in the expression of a set of transcriptional regulatory proteins that are encoded by 'immediate-early' genes. The purpose of this study was to determine whether acute systemic inflammation after lipopolysaccharide injection induces hepatic immediate-early genes that are induced by partial hepatectomy. Methods. Two- to 4-month-old Balb/c mice received intraperitoneal Escherichia coli lipopolysaccharide (0111:B4; 100 μg), and total liver RNA, nuclear protein extracts, or total liver protein lysates were obtained at 0, 1, 3, 12, and 24 hours. RNA blot hybridization analysis was used to determine steady-state messenger RNA levels for c-jun, jun-B, jun-D, c-fos, fos-B, fra-1, nup475, and zif268. Specific nuclear protein- binding activity was determined by gel mobility shift assay. The protein c- Jun was detected by antibody-blocking experiments, and Jun-B was detected by gel supershift assay of the activating protein (AP-1) complex. Steady-state Jun-B levels were determined by immunoblot analysis. Results. Intraperitoneal injection of lipopolysaccharide is followed by induction (from fivefold to 13-fold) of c-jun, jun-B, c-fos, zif268, and nup475 messenger RNAs in the liver. Lipopolysaccharide induced increases in AP-1 and Zif268 consensus DNA- binding activity in mouse liver. The proteins c-Jun and Jun-B are detected in the AP-1 complex after administration of lipopolysaccharide. Conclusions. The induction of hepatic immediate-early genes after lipopolysaccharide is similar to that that follows partial hepatectomy. These transcription factors likely have important roles in the reprogramming of gene expression that leads to the acute-phase response.",
author = "Beauchamp, {R. D.} and John Papaconstantinou and Henderson, {A. M.} and Sheng, {H. M.} and Courtney Townsend and Thompson, {J. C.} and Christou, {N. V.} and Barke, {R. A.} and T. Billiar and Forse, {R. A.} and Baker, {C. C.}",
year = "1994",
language = "English",
volume = "116",
pages = "367--377",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - Activation of hepatic proliferation-associated transcription factors by lipopolysaccharide

AU - Beauchamp, R. D.

AU - Papaconstantinou, John

AU - Henderson, A. M.

AU - Sheng, H. M.

AU - Townsend, Courtney

AU - Thompson, J. C.

AU - Christou, N. V.

AU - Barke, R. A.

AU - Billiar, T.

AU - Forse, R. A.

AU - Baker, C. C.

PY - 1994

Y1 - 1994

N2 - Background. The hepatic acute-phase response is the result of reprogramming of gene expression in the liver. Similar acute-phase responses occur in regenerating liver after partial hepatectomy and are preceded by increases in the expression of a set of transcriptional regulatory proteins that are encoded by 'immediate-early' genes. The purpose of this study was to determine whether acute systemic inflammation after lipopolysaccharide injection induces hepatic immediate-early genes that are induced by partial hepatectomy. Methods. Two- to 4-month-old Balb/c mice received intraperitoneal Escherichia coli lipopolysaccharide (0111:B4; 100 μg), and total liver RNA, nuclear protein extracts, or total liver protein lysates were obtained at 0, 1, 3, 12, and 24 hours. RNA blot hybridization analysis was used to determine steady-state messenger RNA levels for c-jun, jun-B, jun-D, c-fos, fos-B, fra-1, nup475, and zif268. Specific nuclear protein- binding activity was determined by gel mobility shift assay. The protein c- Jun was detected by antibody-blocking experiments, and Jun-B was detected by gel supershift assay of the activating protein (AP-1) complex. Steady-state Jun-B levels were determined by immunoblot analysis. Results. Intraperitoneal injection of lipopolysaccharide is followed by induction (from fivefold to 13-fold) of c-jun, jun-B, c-fos, zif268, and nup475 messenger RNAs in the liver. Lipopolysaccharide induced increases in AP-1 and Zif268 consensus DNA- binding activity in mouse liver. The proteins c-Jun and Jun-B are detected in the AP-1 complex after administration of lipopolysaccharide. Conclusions. The induction of hepatic immediate-early genes after lipopolysaccharide is similar to that that follows partial hepatectomy. These transcription factors likely have important roles in the reprogramming of gene expression that leads to the acute-phase response.

AB - Background. The hepatic acute-phase response is the result of reprogramming of gene expression in the liver. Similar acute-phase responses occur in regenerating liver after partial hepatectomy and are preceded by increases in the expression of a set of transcriptional regulatory proteins that are encoded by 'immediate-early' genes. The purpose of this study was to determine whether acute systemic inflammation after lipopolysaccharide injection induces hepatic immediate-early genes that are induced by partial hepatectomy. Methods. Two- to 4-month-old Balb/c mice received intraperitoneal Escherichia coli lipopolysaccharide (0111:B4; 100 μg), and total liver RNA, nuclear protein extracts, or total liver protein lysates were obtained at 0, 1, 3, 12, and 24 hours. RNA blot hybridization analysis was used to determine steady-state messenger RNA levels for c-jun, jun-B, jun-D, c-fos, fos-B, fra-1, nup475, and zif268. Specific nuclear protein- binding activity was determined by gel mobility shift assay. The protein c- Jun was detected by antibody-blocking experiments, and Jun-B was detected by gel supershift assay of the activating protein (AP-1) complex. Steady-state Jun-B levels were determined by immunoblot analysis. Results. Intraperitoneal injection of lipopolysaccharide is followed by induction (from fivefold to 13-fold) of c-jun, jun-B, c-fos, zif268, and nup475 messenger RNAs in the liver. Lipopolysaccharide induced increases in AP-1 and Zif268 consensus DNA- binding activity in mouse liver. The proteins c-Jun and Jun-B are detected in the AP-1 complex after administration of lipopolysaccharide. Conclusions. The induction of hepatic immediate-early genes after lipopolysaccharide is similar to that that follows partial hepatectomy. These transcription factors likely have important roles in the reprogramming of gene expression that leads to the acute-phase response.

UR - http://www.scopus.com/inward/record.url?scp=0028003722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028003722&partnerID=8YFLogxK

M3 - Article

VL - 116

SP - 367

EP - 377

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 2

ER -