Activation of HIV-1 coreceptor (CXCR4) mediates myelosuppression

Ximena Sanchez, Beth Cousins-Hodges, Tonia Aguilar, Peter Gosselink, Zhijian Lu, Javier Navarro

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Chemokines are cytokines that activate and induce the migration of leukocytes. Stroma-derived factor-1 (SDF-1) is a novel chemokine that blocks the entry of T-tropic HIV-1 mediated by fusin/CXCR4/LESTR (leukocyte-derived seven-transmembrane domain receptor). In this work we demonstrate that SDF-1 triggers increases in intracellular calcium and inhibits the proliferation of myeloid progenitor cell line 32D. By contrast, SDF-1 neither triggers a calcium response nor affects the proliferation of the myeloid progenitor cell line 32D-GR that is deficient in CXCR4. Responsiveness to SDF-1 was rescued by transfection of 32D-GR cells with a cDNA encoding the human CXCR4. The data indicate that SDF-1 induces myelosuppression by activation of CXCR4. The constitutive production of SDF-1 by bone marrow stromal cells argues for a major role of SDF-1 on the regulation of myelopoiesis.

Original languageEnglish (US)
Pages (from-to)27529-27531
Number of pages3
JournalJournal of Biological Chemistry
Volume272
Issue number44
DOIs
StatePublished - Oct 31 1997

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Sanchez, X., Cousins-Hodges, B., Aguilar, T., Gosselink, P., Lu, Z., & Navarro, J. (1997). Activation of HIV-1 coreceptor (CXCR4) mediates myelosuppression. Journal of Biological Chemistry, 272(44), 27529-27531. https://doi.org/10.1074/jbc.272.44.27529