Activation of Human Peripheral Blood Eosinophils by Cytokines in a Comparative Time-Course Proteomic/Phosphoproteomic Study

Kizhake V. Soman, Susan J. Stafford, Konrad Pazdrak, Zheng Wu, Xuemei Luo, Wendy I. White, John E. Wiktorowicz, William Calhoun, Alexander Kurosky

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Activated eosinophils contribute to airway dysfunction and tissue remodeling in asthma and thus are considered to be important factors in asthma pathology. We report here comparative proteomic and phosphoproteomic changes upon activation of eosinophils using eight cytokines individually and in selected cytokine combinations in time-course reactions. Differential protein and phosphoprotein expressions were determined by mass spectrometry after 2-dimensional gel electrophoresis (2DGE) and by LC-MS/MS. We found that each cytokine-stimulation produced significantly different changes in the eosinophil proteome and phosphoproteome, with phosphoproteomic changes being more pronounced and having an earlier onset. Furthermore, we observed that IL-5, GM-CSF, and IL-3 showed the greatest change in protein expression and phosphorylation, and this expression differed markedly from those of the other five cytokines evaluated. Comprehensive univariate and multivariate statistical analyses were employed to evaluate the comparative results. We also monitored eosinophil activation using flow cytometry (FC) analysis of CD69. In agreement with our proteomic studies, FC indicated that IL-5, GM-CSF, and IL-3 were more effective than the other five cytokines studied in stimulating a cell surface CD69 increase indicative of eosinophil activation. Moreover, selected combinations of cytokines revealed proteomic patterns with many proteins in common with single cytokine expression patterns but also showed a greater effect of the two cytokines employed, indicating a more complex signaling pathway that was reflective of a more typical inflammatory pathology.

Original languageEnglish (US)
Pages (from-to)2663-2679
Number of pages17
JournalJournal of Proteome Research
Volume16
Issue number8
DOIs
StatePublished - Aug 4 2017

Fingerprint

Eosinophils
Proteomics
Blood
Chemical activation
Cytokines
Flow cytometry
Interleukin-3
Interleukin-5
Pathology
Granulocyte-Macrophage Colony-Stimulating Factor
Flow Cytometry
Asthma
Phosphorylation
Proteins
Phosphoproteins
Proteome
Electrophoresis
Mass spectrometry
Mass Spectrometry
Multivariate Analysis

Keywords

  • activation
  • asthma
  • cytokines
  • eosinophils
  • flow cytometry
  • mass spectrometry
  • phosphoproteomics
  • proteomics

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry

Cite this

Activation of Human Peripheral Blood Eosinophils by Cytokines in a Comparative Time-Course Proteomic/Phosphoproteomic Study. / Soman, Kizhake V.; Stafford, Susan J.; Pazdrak, Konrad; Wu, Zheng; Luo, Xuemei; White, Wendy I.; Wiktorowicz, John E.; Calhoun, William; Kurosky, Alexander.

In: Journal of Proteome Research, Vol. 16, No. 8, 04.08.2017, p. 2663-2679.

Research output: Contribution to journalArticle

Soman, Kizhake V. ; Stafford, Susan J. ; Pazdrak, Konrad ; Wu, Zheng ; Luo, Xuemei ; White, Wendy I. ; Wiktorowicz, John E. ; Calhoun, William ; Kurosky, Alexander. / Activation of Human Peripheral Blood Eosinophils by Cytokines in a Comparative Time-Course Proteomic/Phosphoproteomic Study. In: Journal of Proteome Research. 2017 ; Vol. 16, No. 8. pp. 2663-2679.
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