Activation of peripheral galanin receptors: Differential effects on nociception

Juan Miguel Jimenez-Andrade, Linda Lundström, Ulla E. Sollenberg, Ülo Langel, Gilberto Castañeda-Hernandez, Susan M. Carlton

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Numerous reports suggest a significant role of peripheral galanin (GAL) in pain transmission; however, due to the lack of selective galanin receptor agonists and antagonists, the role of GAL receptors (GalR1-3) in pain transmission remains unclear. In this study, a new agonist, M617, that preferentially binds to GalR1, a GalR2 agonist (AR-M1896), and a GalR2 antagonist (M871) were tested in the periphery to elucidate the role of peripheral GalR1 and GalR2 in nociception. Ipsilateral, but not contralateral, hindpaw injection of M617 reduced capsaicin (CAP)-induced flinching by ∼ 50%, suggesting that GalR1 activation produces anti-nociception. This anti-nociceptive effect was blocked by intraplantar injection of the non-selective GalR antagonist M35. In contrast ipsilateral, but not contralateral, intraplantar injection of GalR2 agonist AR-M1896 enhanced the CAP-induced nociception (1.7-fold). The GalR2 antagonist M871 blocked the pro-nociceptive effect of AR-M1896 in a dose-dependent manner. This antagonist had no effect on nociceptive behaviors induced by CAP alone. The data demonstrate that activation of peripheral GalR1 results in anti-nociception but activation of peripheral GalR2 produces pro-nociception. Thus, the use of these pharmacological tools may help to elucidate the contribution of GalR subtypes in nociceptive processing, identifying potential drug targets for the treatment of peripheral pain.

    Original languageEnglish (US)
    Pages (from-to)273-280
    Number of pages8
    JournalPharmacology Biochemistry and Behavior
    Volume85
    Issue number1
    DOIs
    StatePublished - Sep 2006

    Fingerprint

    Galanin Receptors
    Nociception
    Capsaicin
    Chemical activation
    Receptor, Galanin, Type 3
    Galanin
    Pain
    Injections
    Processing
    Pharmaceutical Preparations
    galanin (2-11)-amide
    Pharmacology

    Keywords

    • Anti-nociception
    • Capsaicin
    • Inflammatory pain
    • Primary afferent
    • Pro-nociception

    ASJC Scopus subject areas

    • Biochemistry
    • Behavioral Neuroscience
    • Pharmacology

    Cite this

    Jimenez-Andrade, J. M., Lundström, L., Sollenberg, U. E., Langel, Ü., Castañeda-Hernandez, G., & Carlton, S. M. (2006). Activation of peripheral galanin receptors: Differential effects on nociception. Pharmacology Biochemistry and Behavior, 85(1), 273-280. https://doi.org/10.1016/j.pbb.2006.08.008

    Activation of peripheral galanin receptors : Differential effects on nociception. / Jimenez-Andrade, Juan Miguel; Lundström, Linda; Sollenberg, Ulla E.; Langel, Ülo; Castañeda-Hernandez, Gilberto; Carlton, Susan M.

    In: Pharmacology Biochemistry and Behavior, Vol. 85, No. 1, 09.2006, p. 273-280.

    Research output: Contribution to journalArticle

    Jimenez-Andrade, JM, Lundström, L, Sollenberg, UE, Langel, Ü, Castañeda-Hernandez, G & Carlton, SM 2006, 'Activation of peripheral galanin receptors: Differential effects on nociception', Pharmacology Biochemistry and Behavior, vol. 85, no. 1, pp. 273-280. https://doi.org/10.1016/j.pbb.2006.08.008
    Jimenez-Andrade JM, Lundström L, Sollenberg UE, Langel Ü, Castañeda-Hernandez G, Carlton SM. Activation of peripheral galanin receptors: Differential effects on nociception. Pharmacology Biochemistry and Behavior. 2006 Sep;85(1):273-280. https://doi.org/10.1016/j.pbb.2006.08.008
    Jimenez-Andrade, Juan Miguel ; Lundström, Linda ; Sollenberg, Ulla E. ; Langel, Ülo ; Castañeda-Hernandez, Gilberto ; Carlton, Susan M. / Activation of peripheral galanin receptors : Differential effects on nociception. In: Pharmacology Biochemistry and Behavior. 2006 ; Vol. 85, No. 1. pp. 273-280.
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    abstract = "Numerous reports suggest a significant role of peripheral galanin (GAL) in pain transmission; however, due to the lack of selective galanin receptor agonists and antagonists, the role of GAL receptors (GalR1-3) in pain transmission remains unclear. In this study, a new agonist, M617, that preferentially binds to GalR1, a GalR2 agonist (AR-M1896), and a GalR2 antagonist (M871) were tested in the periphery to elucidate the role of peripheral GalR1 and GalR2 in nociception. Ipsilateral, but not contralateral, hindpaw injection of M617 reduced capsaicin (CAP)-induced flinching by ∼ 50{\%}, suggesting that GalR1 activation produces anti-nociception. This anti-nociceptive effect was blocked by intraplantar injection of the non-selective GalR antagonist M35. In contrast ipsilateral, but not contralateral, intraplantar injection of GalR2 agonist AR-M1896 enhanced the CAP-induced nociception (1.7-fold). The GalR2 antagonist M871 blocked the pro-nociceptive effect of AR-M1896 in a dose-dependent manner. This antagonist had no effect on nociceptive behaviors induced by CAP alone. The data demonstrate that activation of peripheral GalR1 results in anti-nociception but activation of peripheral GalR2 produces pro-nociception. Thus, the use of these pharmacological tools may help to elucidate the contribution of GalR subtypes in nociceptive processing, identifying potential drug targets for the treatment of peripheral pain.",
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