Active transport of GSSG from reconstituted erythrocyte ghosts

J. Prchal, Satish Srivastava, E. Beutler

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Human erythrocyte ghosts were loaded with 35S labeled oxidized glutathione (GSSG) and with a sucrose marker, and the transport of GSSG to the suspending medium was studied. GSSG transport from ghosts occurred only when adenosine triphosphate (ATP) was also present in the ghosts, proceeded against a concentration gradient, and was inhibited by fluoride. The rate of transport was dependent upon the intracellular concentration of GSSG. The relationship between GSSG concentration and rate of transport was sigmoidal. Half maximal transport was observed at a GSSG concentration of approximately 9.6 mM. The maximal velocity was estimated to be in the range of 0.27 μmole GSSG per ml of ghosts per hr. These data suggest that the rate of GSSG transport at physiologic concentrations of GSSG is not sufficiently rapid to account for the turnover of glutathione by red cells. It seems more likely that the GSSG transport system serves an emergency function of erythrocytes.

Original languageEnglish (US)
Pages (from-to)111-117
Number of pages7
JournalBlood
Volume46
Issue number1
StatePublished - 1975
Externally publishedYes

Fingerprint

Glutathione Disulfide
Active Biological Transport
Erythrocyte Membrane
Fluorides
Glutathione
Sucrose
Emergencies
Adenosine Triphosphate
Erythrocytes
Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Prchal, J., Srivastava, S., & Beutler, E. (1975). Active transport of GSSG from reconstituted erythrocyte ghosts. Blood, 46(1), 111-117.

Active transport of GSSG from reconstituted erythrocyte ghosts. / Prchal, J.; Srivastava, Satish; Beutler, E.

In: Blood, Vol. 46, No. 1, 1975, p. 111-117.

Research output: Contribution to journalArticle

Prchal, J, Srivastava, S & Beutler, E 1975, 'Active transport of GSSG from reconstituted erythrocyte ghosts', Blood, vol. 46, no. 1, pp. 111-117.
Prchal J, Srivastava S, Beutler E. Active transport of GSSG from reconstituted erythrocyte ghosts. Blood. 1975;46(1):111-117.
Prchal, J. ; Srivastava, Satish ; Beutler, E. / Active transport of GSSG from reconstituted erythrocyte ghosts. In: Blood. 1975 ; Vol. 46, No. 1. pp. 111-117.
@article{2ef51469ca1f44a6aa7e905b160fdfdc,
title = "Active transport of GSSG from reconstituted erythrocyte ghosts",
abstract = "Human erythrocyte ghosts were loaded with 35S labeled oxidized glutathione (GSSG) and with a sucrose marker, and the transport of GSSG to the suspending medium was studied. GSSG transport from ghosts occurred only when adenosine triphosphate (ATP) was also present in the ghosts, proceeded against a concentration gradient, and was inhibited by fluoride. The rate of transport was dependent upon the intracellular concentration of GSSG. The relationship between GSSG concentration and rate of transport was sigmoidal. Half maximal transport was observed at a GSSG concentration of approximately 9.6 mM. The maximal velocity was estimated to be in the range of 0.27 μmole GSSG per ml of ghosts per hr. These data suggest that the rate of GSSG transport at physiologic concentrations of GSSG is not sufficiently rapid to account for the turnover of glutathione by red cells. It seems more likely that the GSSG transport system serves an emergency function of erythrocytes.",
author = "J. Prchal and Satish Srivastava and E. Beutler",
year = "1975",
language = "English (US)",
volume = "46",
pages = "111--117",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "1",

}

TY - JOUR

T1 - Active transport of GSSG from reconstituted erythrocyte ghosts

AU - Prchal, J.

AU - Srivastava, Satish

AU - Beutler, E.

PY - 1975

Y1 - 1975

N2 - Human erythrocyte ghosts were loaded with 35S labeled oxidized glutathione (GSSG) and with a sucrose marker, and the transport of GSSG to the suspending medium was studied. GSSG transport from ghosts occurred only when adenosine triphosphate (ATP) was also present in the ghosts, proceeded against a concentration gradient, and was inhibited by fluoride. The rate of transport was dependent upon the intracellular concentration of GSSG. The relationship between GSSG concentration and rate of transport was sigmoidal. Half maximal transport was observed at a GSSG concentration of approximately 9.6 mM. The maximal velocity was estimated to be in the range of 0.27 μmole GSSG per ml of ghosts per hr. These data suggest that the rate of GSSG transport at physiologic concentrations of GSSG is not sufficiently rapid to account for the turnover of glutathione by red cells. It seems more likely that the GSSG transport system serves an emergency function of erythrocytes.

AB - Human erythrocyte ghosts were loaded with 35S labeled oxidized glutathione (GSSG) and with a sucrose marker, and the transport of GSSG to the suspending medium was studied. GSSG transport from ghosts occurred only when adenosine triphosphate (ATP) was also present in the ghosts, proceeded against a concentration gradient, and was inhibited by fluoride. The rate of transport was dependent upon the intracellular concentration of GSSG. The relationship between GSSG concentration and rate of transport was sigmoidal. Half maximal transport was observed at a GSSG concentration of approximately 9.6 mM. The maximal velocity was estimated to be in the range of 0.27 μmole GSSG per ml of ghosts per hr. These data suggest that the rate of GSSG transport at physiologic concentrations of GSSG is not sufficiently rapid to account for the turnover of glutathione by red cells. It seems more likely that the GSSG transport system serves an emergency function of erythrocytes.

UR - http://www.scopus.com/inward/record.url?scp=0016734110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016734110&partnerID=8YFLogxK

M3 - Article

C2 - 1131422

AN - SCOPUS:0016734110

VL - 46

SP - 111

EP - 117

JO - Blood

JF - Blood

SN - 0006-4971

IS - 1

ER -