Active zone proteins are transported via distinct mechanisms regulated by Par-1 kinase

Kara R. Barber, Julia Tanquary, Keegan Bush, Amanda Shaw, Michael Woodson, Michael Sherman, Yogesh Wairkar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Disruption of synapses underlies a plethora of neurodevelopmental and neurodegenerative disease. Presynaptic specialization called the active zone plays a critical role in the communication with postsynaptic neuron. While the role of many proteins at the active zones in synaptic communication is relatively well studied, very little is known about how these proteins are transported to the synapses. For example, are there distinct mechanisms for the transport of active zone components or are they all transported in the same transport vesicle? Is active zone protein transport regulated? In this report we show that overexpression of Par-1/MARK kinase, a protein whose misregulation has been implicated in Autism spectrum disorders (ASDs) and neurodegenerative disorders, lead to a specific block in the transport of an active zone protein component- Bruchpilot at Drosophila neuromuscular junctions. Consistent with a block in axonal transport, we find a decrease in number of active zones and reduced neurotransmission in flies overexpressing Par-1 kinase. Interestingly, we find that Par-1 acts independently of Tau-one of the most well studied substrates of Par-1, revealing a presynaptic function for Par-1 that is independent of Tau. Thus, our study strongly suggests that there are distinct mechanisms that transport components of active zones and that they are tightly regulated.

Original languageEnglish (US)
Article numbere1006621
JournalPLoS Genetics
Volume13
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Active Biological Transport
phosphotransferases (kinases)
Phosphotransferases
active transport
neurodegenerative diseases
synapse
Neurodegenerative Diseases
Synapses
animal communication
protein
Communication
Transport Vesicles
Proteins
Axonal Transport
protein transport
proteins
Neuromuscular Junction
Protein Transport
Synaptic Transmission
Diptera

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Active zone proteins are transported via distinct mechanisms regulated by Par-1 kinase. / Barber, Kara R.; Tanquary, Julia; Bush, Keegan; Shaw, Amanda; Woodson, Michael; Sherman, Michael; Wairkar, Yogesh.

In: PLoS Genetics, Vol. 13, No. 2, e1006621, 01.02.2017.

Research output: Contribution to journalArticle

Barber, Kara R. ; Tanquary, Julia ; Bush, Keegan ; Shaw, Amanda ; Woodson, Michael ; Sherman, Michael ; Wairkar, Yogesh. / Active zone proteins are transported via distinct mechanisms regulated by Par-1 kinase. In: PLoS Genetics. 2017 ; Vol. 13, No. 2.
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