Activity and regulation of alpha interferon in respiratory syncytial virus and human metapneumovirus experimental infections

Antonieta Guerrero-Plata, Samuel Baron, Joyce S. Poast, Patrick A. Adegboyega, Antonella Casola, Roberto Garofalo

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) cause a similar spectrum of respiratory infections in humans. Classified within the Paramyxoviridae family, Pneumovirinae subfamily, RSV and hMPV present a significant degree of divergence in genome constellation, organization, and protein sequences. RSV has been reported to be a poor inducer of alpha/beta interferons (IFN-α/β) and partially resistant to its antiviral activity. The nature of the innate immune response to hMPV is currently unknown. Herein, an experimental mouse model was used to investigate the interplay between RSV and hMPV infections and IFN-α in the airways. RSV-infected BALB/c mice treated intranasally with either poly-ICLC, a potent inducer of IFN-α, or directly with recombinant IFN-α showed significantly reduced lung viral titers, inflammation, and clinical disease than untreated controls. However, RSV was significantly less sensitive to the antiviral activity of IFN-α than hMPV. Similarly, when the ability to directly induce IFN-α production was assessed, RSV was clearly a weaker inducer of IFN-α than hMPV, as shown by both kinetics and the absolute amount of IFN-α secreted into the bronchoalveolar lavage. To further investigate the putative inhibitory effect of these viruses on IFN-α production, mice were infected for 48 h prior to treatment with poly-ICLC or a specific Toll-like receptor 9 ligand, CpG oligodeoxynucleotides. Strikingly, both poly-ICLC- and CpG-mediated IFN-α production was abrogated by either RSV or MPV infection. These results suggest that a complex interplay between virus-specific and host-mediated responses regulates IFN-α in the lung during infection by members of the Pneumovirinae family.

Original languageEnglish (US)
Pages (from-to)10190-10199
Number of pages10
JournalJournal of Virology
Volume79
Issue number16
DOIs
StatePublished - Aug 2005

Fingerprint

Human metapneumovirus
Metapneumovirus
interferon-alpha
Respiratory Syncytial Viruses
Interferon-alpha
viruses
Infection
Pneumovirinae
infection
Antiviral Agents
Paramyxoviridae
Toll-Like Receptor 9
Viruses
Lung
Aptitude
lungs
Oligodeoxyribonucleotides
Interferon-beta
interferon-beta
Bronchoalveolar Lavage

ASJC Scopus subject areas

  • Immunology

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Activity and regulation of alpha interferon in respiratory syncytial virus and human metapneumovirus experimental infections. / Guerrero-Plata, Antonieta; Baron, Samuel; Poast, Joyce S.; Adegboyega, Patrick A.; Casola, Antonella; Garofalo, Roberto.

In: Journal of Virology, Vol. 79, No. 16, 08.2005, p. 10190-10199.

Research output: Contribution to journalArticle

Guerrero-Plata, Antonieta ; Baron, Samuel ; Poast, Joyce S. ; Adegboyega, Patrick A. ; Casola, Antonella ; Garofalo, Roberto. / Activity and regulation of alpha interferon in respiratory syncytial virus and human metapneumovirus experimental infections. In: Journal of Virology. 2005 ; Vol. 79, No. 16. pp. 10190-10199.
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