Acute bronchial obstruction in sheep

Histopathology and gland cytokine expression

Robert A. Cox, Ann S. Burke, Gloria Oliveras, Perenlei Enkhbaatar, Lillian D. Traber, Joseph B. Zwischenberger, Marc G. Jeschke, Frank C. Schmalstieg, David Herndon, Daniel L. Traber, Hal K. Hawkins

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

An ovine model of smoke inhalation and burn (S+ B) injury models the pathophysiology of these injuries in humans. This study examines the degree of airway obstruction, associated histopathology, and bronchial gland cell expression of cytokines during the first 24 hours after S+ B injury in sheep. Changes in the mean degree of obstruction were limited to the bronchial airways, showing significant increases in obstruction with time, P< .05. At 4 hours after injury, the obstructive material was predominantly mucus, with neutrophils clustered around and within gland acini. At 8 to 24 hours, bronchial obstruction was characterized by increased inflammatory cell accumulation. Immunohistochemical results showed that gland cells constitutively express and secrete interleukin (IL)-1β, and that after injury there is an increase in the percentage of gland cells staining for IL-1α, IL-8, and tumor necrosis factor (TNF)-α, P < .05.

Original languageEnglish (US)
Pages (from-to)819-837
Number of pages19
JournalExperimental Lung Research
Volume31
Issue number9-10
DOIs
StatePublished - Nov 2005

Fingerprint

Interleukin-1
Sheep
Cytokines
Wounds and Injuries
Interleukin-8
Smoke
Tumor Necrosis Factor-alpha
Inhalation Burns
Airway Obstruction
Mucus
Neutrophils
Staining and Labeling

Keywords

  • Airway obstruction
  • Cytokine
  • Inflammation
  • Mucous glands
  • Mucus
  • Neutrophils

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Cox, R. A., Burke, A. S., Oliveras, G., Enkhbaatar, P., Traber, L. D., Zwischenberger, J. B., ... Hawkins, H. K. (2005). Acute bronchial obstruction in sheep: Histopathology and gland cytokine expression. Experimental Lung Research, 31(9-10), 819-837. https://doi.org/10.1080/01902140600574967

Acute bronchial obstruction in sheep : Histopathology and gland cytokine expression. / Cox, Robert A.; Burke, Ann S.; Oliveras, Gloria; Enkhbaatar, Perenlei; Traber, Lillian D.; Zwischenberger, Joseph B.; Jeschke, Marc G.; Schmalstieg, Frank C.; Herndon, David; Traber, Daniel L.; Hawkins, Hal K.

In: Experimental Lung Research, Vol. 31, No. 9-10, 11.2005, p. 819-837.

Research output: Contribution to journalArticle

Cox, RA, Burke, AS, Oliveras, G, Enkhbaatar, P, Traber, LD, Zwischenberger, JB, Jeschke, MG, Schmalstieg, FC, Herndon, D, Traber, DL & Hawkins, HK 2005, 'Acute bronchial obstruction in sheep: Histopathology and gland cytokine expression', Experimental Lung Research, vol. 31, no. 9-10, pp. 819-837. https://doi.org/10.1080/01902140600574967
Cox, Robert A. ; Burke, Ann S. ; Oliveras, Gloria ; Enkhbaatar, Perenlei ; Traber, Lillian D. ; Zwischenberger, Joseph B. ; Jeschke, Marc G. ; Schmalstieg, Frank C. ; Herndon, David ; Traber, Daniel L. ; Hawkins, Hal K. / Acute bronchial obstruction in sheep : Histopathology and gland cytokine expression. In: Experimental Lung Research. 2005 ; Vol. 31, No. 9-10. pp. 819-837.
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