Acute dichloroacetate administration increases skeletal muscle free glutamine concentrations after burn injury

Arny A. Ferrando, David L. Chinkes, Steven Wolf, Sina Matin, David Herndon, Robert R. Wolfe

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: To investigate the hypothesis that the stimulation of pyruvate oxidation by dichloroacetate (DCA) administration would increase the level of intramuscular glutamine in severely burned patients. Summary Background Data: The level of intramuscular glutamine decreases in response to severe injury, and the rate of intramuscular glycolysis and pyruvate oxidation is elevated. Intramuscular glutamine concentrations have been correlated to muscle protein synthesis. Methods: Six studies were conducted on five patients with burns >40% total body surface area. Patients were studied in the fed state during an 8-hour stable isotope infusion. After 5 hours, DCA (30 mg/kg) was administered for 30 minutes. Results: Analysis of muscle biopsy samples taken at 5 and 8 hours of the study revealed a 32% increase in intracellular glutamine levels after DCA administration. Increased intracellular glutamine concentrations did not affect skeletal muscle protein synthesis as determined by a three-pool arteriovenous model or by the direct incorporation of isotope into skeletal muscle protein, DCA administration resulted in a decrease in plasma lactate but no change in alanine de novo synthesis or intracellular concentration. Conclusions: These results suggest that acute DCA administration can increase intramuscular glutamine concentration, but that this acute elevation does not affect muscle protein metabolism.

Original languageEnglish (US)
Pages (from-to)249-256
Number of pages8
JournalAnnals of Surgery
Volume228
Issue number2
DOIs
StatePublished - Aug 1998

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Glutamine
Skeletal Muscle
Muscle Proteins
Wounds and Injuries
Pyruvic Acid
Isotopes
Body Surface Area
Glycolysis
Burns
Alanine
Lactic Acid
Biopsy
Muscles

ASJC Scopus subject areas

  • Surgery

Cite this

Acute dichloroacetate administration increases skeletal muscle free glutamine concentrations after burn injury. / Ferrando, Arny A.; Chinkes, David L.; Wolf, Steven; Matin, Sina; Herndon, David; Wolfe, Robert R.

In: Annals of Surgery, Vol. 228, No. 2, 08.1998, p. 249-256.

Research output: Contribution to journalArticle

Ferrando, Arny A. ; Chinkes, David L. ; Wolf, Steven ; Matin, Sina ; Herndon, David ; Wolfe, Robert R. / Acute dichloroacetate administration increases skeletal muscle free glutamine concentrations after burn injury. In: Annals of Surgery. 1998 ; Vol. 228, No. 2. pp. 249-256.
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