TY - JOUR
T1 - Acute monocyte pro inflammatory response predicts higher positive to negative acute phase reactants ratio and severe hemostatic derangement in dengue fever
AU - Choudhuri, Subhadip
AU - Chowdhury, Imran Hussain
AU - Saha, Avijit
AU - Mitra, Bhaskar
AU - Dastidar, Rinini
AU - Roy, Pijush Kanti
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/10
Y1 - 2021/10
N2 - Purpose: The present study was intended to investigate whether monocyte immune activation shapes plasma positive to negative acute phase reactants (APRs) ratio and predicts disease severity in dengue infection. Methods: Serum level of ferritin, ceruloplasmin and transferrin was measured by means of electrochemiluminescence and immunoturbidimetry, respectively. Gene expression and plasma level for TNF-α, IL-6 and IL1-β was measured by means of RT-qPCR and ELISA. Results: A significant increased serum ferritin to transferrin [6.6 (3–11.7) vs 3.4 (1.9–6.1)] and ceruloplasmin to transferrin ratio [0.48 (0.21–0.87) vs 0.22 (0.13–0.43)] has been detected among the subjects with secondary dengue infection (SDENI) compared to primarily infected (PDENI) subjects (P < 0.001). Significant increased expression for CD14+ monocyte TNF-α, IL-6 and IL-1β has been detected in SDENI patients (vs PDENI and control, P < 0.001). Plasma ferritin to transferrin ratio was found in a significant association with high level of plasma TNF-α [ρ = 0.6522, 95% CI (0.4714–0.7805)], IL-6 [ρ = 0.6181, 95% CI (0.4257–0.7571)] and IL- 1β [ρ = 0.4119, 95% CI (0.1689–0.6077)] level among SDENI patients at 5th day time point after progression of the disease, with significantly low platelet [P < 0.001] and prolonging prothrombin time [P < 0.001] compared to control and PDENI subjects, respectively. Conclusion: Acute proinflammatory cytokine response is significantly associated with increased positive to negative APRs ratio in SDENI patients, which predicts intense immune activation, and renders SDENI patients extremely susceptible to hemostatic derangement.
AB - Purpose: The present study was intended to investigate whether monocyte immune activation shapes plasma positive to negative acute phase reactants (APRs) ratio and predicts disease severity in dengue infection. Methods: Serum level of ferritin, ceruloplasmin and transferrin was measured by means of electrochemiluminescence and immunoturbidimetry, respectively. Gene expression and plasma level for TNF-α, IL-6 and IL1-β was measured by means of RT-qPCR and ELISA. Results: A significant increased serum ferritin to transferrin [6.6 (3–11.7) vs 3.4 (1.9–6.1)] and ceruloplasmin to transferrin ratio [0.48 (0.21–0.87) vs 0.22 (0.13–0.43)] has been detected among the subjects with secondary dengue infection (SDENI) compared to primarily infected (PDENI) subjects (P < 0.001). Significant increased expression for CD14+ monocyte TNF-α, IL-6 and IL-1β has been detected in SDENI patients (vs PDENI and control, P < 0.001). Plasma ferritin to transferrin ratio was found in a significant association with high level of plasma TNF-α [ρ = 0.6522, 95% CI (0.4714–0.7805)], IL-6 [ρ = 0.6181, 95% CI (0.4257–0.7571)] and IL- 1β [ρ = 0.4119, 95% CI (0.1689–0.6077)] level among SDENI patients at 5th day time point after progression of the disease, with significantly low platelet [P < 0.001] and prolonging prothrombin time [P < 0.001] compared to control and PDENI subjects, respectively. Conclusion: Acute proinflammatory cytokine response is significantly associated with increased positive to negative APRs ratio in SDENI patients, which predicts intense immune activation, and renders SDENI patients extremely susceptible to hemostatic derangement.
KW - Dengue
KW - Monocyte
KW - Negative acute phase reactants
KW - Positive acute phase reactants
KW - Proinflammatory cytokine
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U2 - 10.1016/j.cyto.2021.155644
DO - 10.1016/j.cyto.2021.155644
M3 - Article
C2 - 34298483
AN - SCOPUS:85110649361
SN - 1043-4666
VL - 146
JO - Cytokine
JF - Cytokine
M1 - 155644
ER -