Acute pancreatitis results in induction of heat shock proteins 70 and 27 and heat shock factor-1

Richard T. Ethridge, Richard A. Ehlers, Mark Hellmich, Srinivasan Rajaraman, B. Mark Evers

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Heat shock proteins (HSPs) 70 and 27 are stress-responsive proteins that are important for cell survival after injury; the expression of these HSPs is regulated primarily by the transcription factor heat shock factor-1 (HSF-1). The purpose of this study was to determine the effect of acute pancreatitis on pancreatic HSPs (70, 27, 60, and 90) expression and to assess potential mechanisms for HSP induction using a murine model of cemlein-induced pancreatitis. We found an increase of both HSP70 and HSP27 levels with expression noted throughout the pancreas after induction of pancreatitis. HSP60 and HSP90 levels were constitutively expressed in the pancreas and did not significantly change with acute pancreatitis. HSF-1 DNA binding activity increased in accordance with increased HSP expression. We conclude that acute pancreatitis results in a marked increase in the expression of HSP70 and HSP27. Furthermore, the induction of HSP70 and HSP27 expression was associated with a concomitant increase in HSF-1 binding activity. The increased expression of both HSP70 and HSP27 noted with pancreatic inflammation may confer a protective effect for the remaining acini after acute pancreatitis.

Original languageEnglish (US)
Pages (from-to)248-256
Number of pages9
JournalPancreas
Volume21
Issue number3
DOIs
StatePublished - 2000

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HSP27 Heat-Shock Proteins
HSP70 Heat-Shock Proteins
Pancreatitis
Shock
Hot Temperature
Heat-Shock Proteins
Pancreas
Cell Survival
Inflammation
DNA
Wounds and Injuries

Keywords

  • Heat shock factor
  • Heat shock proteins
  • Pancreatitis

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology

Cite this

Acute pancreatitis results in induction of heat shock proteins 70 and 27 and heat shock factor-1. / Ethridge, Richard T.; Ehlers, Richard A.; Hellmich, Mark; Rajaraman, Srinivasan; Evers, B. Mark.

In: Pancreas, Vol. 21, No. 3, 2000, p. 248-256.

Research output: Contribution to journalArticle

Ethridge, Richard T. ; Ehlers, Richard A. ; Hellmich, Mark ; Rajaraman, Srinivasan ; Evers, B. Mark. / Acute pancreatitis results in induction of heat shock proteins 70 and 27 and heat shock factor-1. In: Pancreas. 2000 ; Vol. 21, No. 3. pp. 248-256.
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