Acute response of net muscle protein balance reflects 24-h balance after exercise and amino acid ingestion

Kevin D. Tipton, Elisabet Borsheim, Steven E. Wolf, Arthur P. Sanford, Robert R. Wolfe

Research output: Contribution to journalArticlepeer-review

163 Scopus citations


The purpose of this study was to determine if the acute anabolic muscle response to resistance exercise and essential amino acids (EAA) reflects the response over 24 h. Seven subjects participated in the following two 24-h studies: 1) resting (REST) and 2) rest plus resistance exercise and consumption of EAA (ES). Net balance (NB) across the leg was determined for four amino acids. [13C6]phenylalanine was infused to determine mixed muscle fractional synthetic rate (FSR). Twenty-four-hour FSR was significantly greater for ES than for REST (P = 0.003). Exchange of phenylalanine across the leg was -194 ± 74 (SE) mg for ES and -371 ± 88 mg for REST (P = 0.07) over 24 h and 229 ± 42 mg (ES) and 28 ± 15 mg (REST; P < 0.01) over 3 h corresponding to exercise and EAA consumption for ES. The difference in phenylalanine exchange between REST and ES was not different for measurements over 24 and 3 h. Increases in NB during ES were primarily the result of increases in protein synthesis. Results for other amino acids were similar. The acute anabolic response of muscle to EAA intake and exercise is additive to the response at rest and thus reflects the 24-h response.

Original languageEnglish (US)
Pages (from-to)E76-E89
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number1 47-1
StatePublished - Jan 1 2003


  • Muscle protein breakdown
  • Muscle protein synthesis
  • Stable isotopic tracers

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)


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