Adaptation of yellow fever virus 17D to Vero cells is associated with mutations in structural and non-structural protein genes

David Beasley, Merribeth Morin, Ashley R. Lamb, Edward Hayman, Douglas M. Watts, Cynthia K. Lee, Dennis W. Trent, Thomas P. Monath

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Serial passaging of yellow fever virus 17D in Vero cells was employed to derive seed material for a novel inactivated vaccine, XRX-001. Two independent passaging series identified a novel lysine to arginine mutation at amino acid 160 of the envelope protein, a surface-exposed residue in structural domain I. A third passage series resulted in an isoleucine to methionine mutation at residue 113 of the NS4B protein, a central membrane spanning region of the protein which has previously been associated with Vero cell adaptation of other mosquito-borne flaviviruses. These studies confirm that flavivirus adaptation to growth in Vero cells can be mediated by structural or non-structural protein mutations.

Original languageEnglish (US)
Pages (from-to)280-284
Number of pages5
JournalVirus Research
Volume176
Issue number1-2
DOIs
StatePublished - Sep 2013

Fingerprint

Yellow fever virus
Vero Cells
Flavivirus
Mutation
Inactivated Vaccines
Proteins
Isoleucine
Culicidae
Methionine
Lysine
Arginine
Seeds
Membrane Proteins
Amino Acids
Membranes
Growth

Keywords

  • Vaccine
  • Vero cells
  • Yellow fever virus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Adaptation of yellow fever virus 17D to Vero cells is associated with mutations in structural and non-structural protein genes. / Beasley, David; Morin, Merribeth; Lamb, Ashley R.; Hayman, Edward; Watts, Douglas M.; Lee, Cynthia K.; Trent, Dennis W.; Monath, Thomas P.

In: Virus Research, Vol. 176, No. 1-2, 09.2013, p. 280-284.

Research output: Contribution to journalArticle

Beasley, David ; Morin, Merribeth ; Lamb, Ashley R. ; Hayman, Edward ; Watts, Douglas M. ; Lee, Cynthia K. ; Trent, Dennis W. ; Monath, Thomas P. / Adaptation of yellow fever virus 17D to Vero cells is associated with mutations in structural and non-structural protein genes. In: Virus Research. 2013 ; Vol. 176, No. 1-2. pp. 280-284.
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