Additional studies have been carried out on erythrocytes containing an unstable hemoglobin (Sabine). Increased levels of hexokinase and glyceraldehyde phosphate dehydrogenase, together with a high intracellular inorganic phosphate level and a high ADP ATP ratio, account for the very rapid glycolytic rate in these cells. The high intracellular inorganic phosphate level appears to be a consequence of increased uptake of phosphate into the cells, with uptake coupled to activities of two membrane enzymes, glyceraldehyde phosphate dehydrogenase and phosphoglycerate kinase. In vitro incubation studies over a period of 14 hours indicate that these cells maintain intracellular K+ and Na+ levels satisfactorily. These studies do not, however, indicate whether rates of influx or efflux of these cations are modified in these abnormal erythrocytes.
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