Adenosine kinase as a new selective marker in somatic cell genetics

Isolation of adenosine kinase-deficient mouse cell lines and human-mouse hybrid cell lines containing adenosine kinase

Teh sheng Chan, Richard P. Creagan, M. Patricia Reardon

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A new selective system for isolating somatic cell hybrids, using adenosine kinase as the selective marker, has been developed. The selective medium for forward selection (to select for cells containing adenosine kinase) contains alanosine, adenosine and uridine. To survive in the presence of alanosine, cells must have adenosine kinase in order to utilize exogenous adenosine as the sole source of AMP. Uridine is added to the selective medium to prevent the toxic effects of adenosine on cultured mammalian cells. The selective medium for reverse selection (to select for cells lacking adenosine kinase) contains 2-fluoroadenosine, an analogue of adenosine, which is converted to a toxic nucleotide by the action of adenosine kinase. Mouse mutant cell lines deficient in adenosine kinase have been derived. Human-mouse hybrid cells containing the kinase have been prepared from one of these mutant lines. Karyotype data of these hybrid lines and their adenosine kinase-minus sublines are consistent with assignment by others of the human gene for adenosine kinase on chromosome 10.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalSomatic Cell Genetics
Volume4
Issue number1
DOIs
StatePublished - Jan 1978
Externally publishedYes

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Adenosine Kinase
Cell Separation
Hybrid Cells
Cell Line
alanosine
Adenosine
Uridine
Toxic Actions
Chromosomes, Human, Pair 10
Poisons
Adenosine Monophosphate
Karyotype
Cultured Cells
Phosphotransferases
Nucleotides

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Adenosine kinase as a new selective marker in somatic cell genetics : Isolation of adenosine kinase-deficient mouse cell lines and human-mouse hybrid cell lines containing adenosine kinase. / Chan, Teh sheng; Creagan, Richard P.; Reardon, M. Patricia.

In: Somatic Cell Genetics, Vol. 4, No. 1, 01.1978, p. 1-12.

Research output: Contribution to journalArticle

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