Adenosine kinase deficiency in tritiated deoxyadenosine-resistant mouse S49 lymphoma cell lines

K. Jagannadha Sastry, Cecilia Huang, Teh sheng Chan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mutant sublines were derived of S49 mouse T-lymphoma cells that were resistant to tritiated deoxyadenosine. Twenty-five isolates that were selected in 1 μCi/ml of the nucleoside were cross-resistant to 6-thioguanine, were sensitive to HAT (hypoxanthine, aminopterin, and thymidine), and contained less than 1% of hypoxanthine phosphoribosyltransferase activity in wildtype cells. One of the mutant clones, S49-dA2, was further subjected to selection in a medium containing 2 μCi/ml tritiated deoxyadenosine and 1 μg/ml deoxycoformycin, an inhibitor of adenosine deaminase. All resistant subclones were cross-resistant to tubercidin, 6-methylmercaptopurine riboside, and arabinosyladenine. One of the subclones, S49-12, was completely devoid of adenosine kinase and was partially deficient in deoxyadenosine kinase. This subclone, however, contained wild-type levels of deoxycytidine kinase. DEAE chromatography of the wild-type cell extracts revealed two deoxyadenosine phosphorylating activities, one of which coeluted with adenosine kinase and was the enzyme missing in S49-12. The other species phosphorylated both deoxyadenosine and deoxycytidine, of which deoxycytidine was the preferred substrate.

Original languageEnglish (US)
Pages (from-to)765-777
Number of pages13
JournalBiochemical Genetics
Volume25
Issue number11-12
DOIs
StatePublished - Dec 1987

Fingerprint

adenosine kinase
Adenosine Kinase
lymphoma
Lymphoma
hypoxanthine
Cells
cell lines
Cell Line
Deoxycytidine
deoxyadenosine kinase
mice
phosphotransferases (kinases)
aminopterin
adenosine deaminase
chromatography
Methylthioinosine
clone
inhibitor
mutants
Tubercidin

Keywords

  • deoxyadenosine kinase
  • deoxycytidine kinase
  • nucleoside analogues
  • tritium suicide

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Adenosine kinase deficiency in tritiated deoxyadenosine-resistant mouse S49 lymphoma cell lines. / Jagannadha Sastry, K.; Huang, Cecilia; Chan, Teh sheng.

In: Biochemical Genetics, Vol. 25, No. 11-12, 12.1987, p. 765-777.

Research output: Contribution to journalArticle

Jagannadha Sastry, K. ; Huang, Cecilia ; Chan, Teh sheng. / Adenosine kinase deficiency in tritiated deoxyadenosine-resistant mouse S49 lymphoma cell lines. In: Biochemical Genetics. 1987 ; Vol. 25, No. 11-12. pp. 765-777.
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