Adenosine modulates n-methyl-d-aspartate-stimulated hippocampal nitric oxide production in vivo

Anish Bhardwaj, Frances J. Northington, Raymond C. Koehler, Theodore Stiefel, Daniel F. Hanley, Richard J. Traystman

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43 Scopus citations

Abstract

and PurposeAdenosine acts presynaptically to inhibit release of excitatory amino acids (EAAs) and is thus considered to be neuroprotective. Because EAA-stimulated synthesis of nitric oxide (NO) may play an important role in long-term potentiation and excitotoxic-mediated injury, we tested the hypotheses that adenosine agonists attenuate basal and EAA-induced NO production in the hippocampus in vivo and that adenosine A1 receptors mediate this response. MethodsMicrodialysis probes were placed bilaterally into the CA3 region of the hippocampus of adult Sprague-Dawley rats under pentobarbital anesthesia. Probes were perfused for 5 hours with artificial cerebrospinal fluid containing 3 mu mol/L [sup 14 C]L-arginine. Recovery of [sup 14 C]L-citrulline in the effluent was used as a marker of NO production. In 10 groups of rats, time-dependent increases in [sup 14 C]L-citrulline recovery were compared between right- and left-sided probes perfused with various combinations of N-methyl-D-aspartate (NMDA), adenosine agonists, adenosine antagonists, and the NO synthase inhibitor Nomega -nitro-L-arginine methyl ester (L-NAME). ResultsRecovery of [sup 14 C]L-citrulline during perfusion with artificial cerebrospinal fluid progressively increased to 141 plus minus 27 fmol/min (plus minus SEM) over 5 hours. Contralateral perfusion with 1 mmol/L NMDA augmented [sup 14 C]L-citrulline recovery to 317 plus minus 62 fmol/min. Perfusion of 1 mmol/L L-NAME with NMDA inhibited [sup 14 C]L-citrulline recovery compared with NMDA alone. Perfusion with 0.1 mmol/L 2-chloroadenosine attenuated basal as well as NMDA-enhanced [sup 14 C]L-citrulline recovery. This action of 2-chloroadenosine was reversed by infusion of 0.1 mmol/L 8-cyclopentyl-1,3-dipropylxanthine, a specific A1 receptor antagonist. Infusion of 0.1 mmol/L (2S)-N6-[2-endo-norboryl]adenosine, a specific A1 receptor agonist, also attenuated the 0.1 mmol/L and 1 mmol/L NMDA-enhanced [sup 14 C]L-citrulline recovery. ConclusionsUsing an indirect method of assessing NO production in vivo, these data are consistent with in vitro results showing that NMDA receptor stimulation enhances NO production. Furthermore, we conclude that stimulation of A1 receptors can attenuate the basal as well as NMDA-induced production of NO. Because NMDA receptor stimulation amplifies glutamate release, our data are consistent with presynaptic A1 receptor-mediated inhibition of EAA release and consequent downregulation of NO production.

Original languageEnglish (US)
Pages (from-to)1627-1633
Number of pages7
JournalStroke
Volume26
Issue number9
DOIs
StatePublished - Sep 1995
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

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    Bhardwaj, A., Northington, F. J., Koehler, R. C., Stiefel, T., Hanley, D. F., & Traystman, R. J. (1995). Adenosine modulates n-methyl-d-aspartate-stimulated hippocampal nitric oxide production in vivo. Stroke, 26(9), 1627-1633. https://doi.org/10.1161/01.STR.26.9.1627