Adenosine receptor agonists inhibit Macrophage Inflammatory Protein (MIP)-1α production in immunostimulated macrophages

T. P. Shanley, G. Haskó, G. Egnaczyk, A. L. Salzman, Csaba Szabo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ligands of the various adenosine receptor subtypes have been shown to modulate the production of pro-and anti-inflammatory cytokines. Macrophage inflammatory protein (MP) 1α is a chemokine which enhances neutrophil recruitment into inflammatory sites. Here we evaluated the effect of various ligands of the adenosine receptors on MIP-1α production in immunostimulated RAW macrophages in vitro. The A3 receptor agonist N6-(3-iodobenzyl)-adenosine-5′-N-methyluronamide (IB-MECA), and, less potently, the A2 receptor agonist CGS-21680 (1-200 μM) dose-dependently suppressed the production of MIP-1α, whereas the selective A1 receptor agonist 2-chloro-N6-cyclopentyladenosine was ineffective, and adenosine was a weak inhibitor. The inhibition by the adenosine agonists was associated with suppression of MIP-1α steady-state mRNA levels. These data demonstrate that activation of the A3, and to a lesser extent A2 adenosine receptors suppresses MIP-1α expression in immunostimulated macrophages. This effect may contribute to the immunosuppressive properties of adenosine or adenosine receptor ligands in inflammatory conditions.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

Fingerprint

Purinergic P1 Receptor Agonists
Macrophage Inflammatory Proteins
Macrophages
adenosine
agonists
macrophages
Adenosine
Purinergic P1 Receptors
receptors
Ligands
Adenosine A2 Receptors
Neutrophil Infiltration
Immunosuppressive Agents
Chemokines
varespladib methyl
immunosuppressive agents
Anti-Inflammatory Agents
Chemical activation
macrophage inflammatory proteins
Cytokines

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Adenosine receptor agonists inhibit Macrophage Inflammatory Protein (MIP)-1α production in immunostimulated macrophages. / Shanley, T. P.; Haskó, G.; Egnaczyk, G.; Salzman, A. L.; Szabo, Csaba.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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AU - Salzman, A. L.

AU - Szabo, Csaba

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