Adenovirus-based vaccine prevents pneumonia in ferrets challenged with the SARS coronavirus and stimulates robust immune responses in macaques

Gary P. Kobinger, Joanita M. Figueredo, Thomas Rowe, Yan Zhi, Guangping Gao, Julio C. Sanmiguel, Peter Bell, Nelson A. Wivel, Lois A. Zitzow, Douglas B. Flieder, Robert J. Hogan, James M. Wilson

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

A ferret model of severe acute respiratory syndrome (SARS)-CoV infection was used to evaluate the efficacy of an adenovirus vaccine. Animals were subjected to heterologous prime-boost using vectors from human serotype 5 and chimpanzee derived adenoviruses (human AdHu5 and chimpanzee AdC7) expressing spike protein followed by intranasal challenge with SARS-CoV. Vaccination led to a substantial reduction in viral load and prevented the severe pneumonia seen in unvaccinated animals. The same prime-boost strategy was effective in rhesus macaques in eliciting SARS-CoV specific immune responses. These data indicate that a heterologous adenovirus-based prime-boost vaccine strategy could safely stimulate strong immunity that may be needed for complete protection against SARS-CoV infection.

Original languageEnglish (US)
Pages (from-to)5220-5231
Number of pages12
JournalVaccine
Volume25
Issue number28
DOIs
StatePublished - Jul 9 2007
Externally publishedYes

Keywords

  • Adenovirus
  • SARS
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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