Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1

C. Wayne Smith, Mark L. Entman, Caryl L. Lane, Arthur L. Beaudet, Theresa I. Ty, Keith Youker, Hal K. Hawkins, D. C. Anderson

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

The adhesiveness of isolated canine cardiac myocytes for neutrophils is greatly increased by stimulation with cytokines such as tumor necrosis factor α (TNFα). Since this adhesion is significantly inhibited by an anti-CD 18 MAb, experiments were performed to test the hypothesis that the newly expressed adhesion molecule on the cardiac myocytes was intercellular adhesion molecule-1 (ICAM-1). A newly developed MAb, CL18/6, was found to exhibit the functional and binding characteristics with canine neutrophils and canine jugular vein endothelial cells expected of an antibody recognizing ICAM-1. MAb CL18/6 also bound to isolated cardiac myocytes after stimulation of the myocytes with cytokines, and it blocked by > 90% the adhesion of neutrophils to stimulated myocytes. A partial cDNA clone for canine ICAM-1 was isolated, and ICAM-1 mRN A was found to be increased in both endothelial cells and cardiac myocytes after cytokine stimulation. Cytokines that both increased the CL18/6-inhibitable adhesion of neutrophils to myocytes and induced expression of ICAM-1 were IL-1β, TNFα, and LPS. These results are consistent with the conclusion that canine endothelial cells and cardiac myocytes express ICAM-1 in response to cytokine stimulation, and that ICAM-1 functions as an adhesive molecule for neutrophils on both cell types.

Original languageEnglish (US)
Pages (from-to)1216-1223
Number of pages8
JournalJournal of Clinical Investigation
Volume88
Issue number4
StatePublished - 1991
Externally publishedYes

Fingerprint

Intercellular Adhesion Molecule-1
Cardiac Myocytes
Canidae
Neutrophils
Cytokines
Muscle Cells
Endothelial Cells
Tumor Necrosis Factor-alpha
Adhesiveness
Jugular Veins
In Vitro Techniques
Interleukin-1
Adhesives
Complementary DNA
Clone Cells
Antibodies

Keywords

  • Adhesion cytokines
  • ICAM-1
  • Myocyte
  • Neutrophil

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Smith, C. W., Entman, M. L., Lane, C. L., Beaudet, A. L., Ty, T. I., Youker, K., ... Anderson, D. C. (1991). Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1. Journal of Clinical Investigation, 88(4), 1216-1223.

Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1. / Smith, C. Wayne; Entman, Mark L.; Lane, Caryl L.; Beaudet, Arthur L.; Ty, Theresa I.; Youker, Keith; Hawkins, Hal K.; Anderson, D. C.

In: Journal of Clinical Investigation, Vol. 88, No. 4, 1991, p. 1216-1223.

Research output: Contribution to journalArticle

Smith, CW, Entman, ML, Lane, CL, Beaudet, AL, Ty, TI, Youker, K, Hawkins, HK & Anderson, DC 1991, 'Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1', Journal of Clinical Investigation, vol. 88, no. 4, pp. 1216-1223.
Smith, C. Wayne ; Entman, Mark L. ; Lane, Caryl L. ; Beaudet, Arthur L. ; Ty, Theresa I. ; Youker, Keith ; Hawkins, Hal K. ; Anderson, D. C. / Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1. In: Journal of Clinical Investigation. 1991 ; Vol. 88, No. 4. pp. 1216-1223.
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