Abstract
The adhesiveness of isolated canine cardiac myocytes for neutrophils is greatly increased by stimulation with cytokines such as tumor necrosis factor α (TNFα). Since this adhesion is significantly inhibited by an anti-CD 18 MAb, experiments were performed to test the hypothesis that the newly expressed adhesion molecule on the cardiac myocytes was intercellular adhesion molecule-1 (ICAM-1). A newly developed MAb, CL18/6, was found to exhibit the functional and binding characteristics with canine neutrophils and canine jugular vein endothelial cells expected of an antibody recognizing ICAM-1. MAb CL18/6 also bound to isolated cardiac myocytes after stimulation of the myocytes with cytokines, and it blocked by > 90% the adhesion of neutrophils to stimulated myocytes. A partial cDNA clone for canine ICAM-1 was isolated, and ICAM-1 mRN A was found to be increased in both endothelial cells and cardiac myocytes after cytokine stimulation. Cytokines that both increased the CL18/6-inhibitable adhesion of neutrophils to myocytes and induced expression of ICAM-1 were IL-1β, TNFα, and LPS. These results are consistent with the conclusion that canine endothelial cells and cardiac myocytes express ICAM-1 in response to cytokine stimulation, and that ICAM-1 functions as an adhesive molecule for neutrophils on both cell types.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1216-1223 |
| Number of pages | 8 |
| Journal | Journal of Clinical Investigation |
| Volume | 88 |
| Issue number | 4 |
| State | Published - 1991 |
| Externally published | Yes |
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Keywords
- Adhesion cytokines
- ICAM-1
- Myocyte
- Neutrophil
ASJC Scopus subject areas
- Medicine(all)
Cite this
Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1. / Smith, C. Wayne; Entman, Mark L.; Lane, Caryl L.; Beaudet, Arthur L.; Ty, Theresa I.; Youker, Keith; Hawkins, Hal K.; Anderson, D. C.
In: Journal of Clinical Investigation, Vol. 88, No. 4, 1991, p. 1216-1223.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1
AU - Smith, C. Wayne
AU - Entman, Mark L.
AU - Lane, Caryl L.
AU - Beaudet, Arthur L.
AU - Ty, Theresa I.
AU - Youker, Keith
AU - Hawkins, Hal K.
AU - Anderson, D. C.
PY - 1991
Y1 - 1991
N2 - The adhesiveness of isolated canine cardiac myocytes for neutrophils is greatly increased by stimulation with cytokines such as tumor necrosis factor α (TNFα). Since this adhesion is significantly inhibited by an anti-CD 18 MAb, experiments were performed to test the hypothesis that the newly expressed adhesion molecule on the cardiac myocytes was intercellular adhesion molecule-1 (ICAM-1). A newly developed MAb, CL18/6, was found to exhibit the functional and binding characteristics with canine neutrophils and canine jugular vein endothelial cells expected of an antibody recognizing ICAM-1. MAb CL18/6 also bound to isolated cardiac myocytes after stimulation of the myocytes with cytokines, and it blocked by > 90% the adhesion of neutrophils to stimulated myocytes. A partial cDNA clone for canine ICAM-1 was isolated, and ICAM-1 mRN A was found to be increased in both endothelial cells and cardiac myocytes after cytokine stimulation. Cytokines that both increased the CL18/6-inhibitable adhesion of neutrophils to myocytes and induced expression of ICAM-1 were IL-1β, TNFα, and LPS. These results are consistent with the conclusion that canine endothelial cells and cardiac myocytes express ICAM-1 in response to cytokine stimulation, and that ICAM-1 functions as an adhesive molecule for neutrophils on both cell types.
AB - The adhesiveness of isolated canine cardiac myocytes for neutrophils is greatly increased by stimulation with cytokines such as tumor necrosis factor α (TNFα). Since this adhesion is significantly inhibited by an anti-CD 18 MAb, experiments were performed to test the hypothesis that the newly expressed adhesion molecule on the cardiac myocytes was intercellular adhesion molecule-1 (ICAM-1). A newly developed MAb, CL18/6, was found to exhibit the functional and binding characteristics with canine neutrophils and canine jugular vein endothelial cells expected of an antibody recognizing ICAM-1. MAb CL18/6 also bound to isolated cardiac myocytes after stimulation of the myocytes with cytokines, and it blocked by > 90% the adhesion of neutrophils to stimulated myocytes. A partial cDNA clone for canine ICAM-1 was isolated, and ICAM-1 mRN A was found to be increased in both endothelial cells and cardiac myocytes after cytokine stimulation. Cytokines that both increased the CL18/6-inhibitable adhesion of neutrophils to myocytes and induced expression of ICAM-1 were IL-1β, TNFα, and LPS. These results are consistent with the conclusion that canine endothelial cells and cardiac myocytes express ICAM-1 in response to cytokine stimulation, and that ICAM-1 functions as an adhesive molecule for neutrophils on both cell types.
KW - Adhesion cytokines
KW - ICAM-1
KW - Myocyte
KW - Neutrophil
UR - http://www.scopus.com/inward/record.url?scp=0026043977&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026043977&partnerID=8YFLogxK
M3 - Article
VL - 88
SP - 1216
EP - 1223
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
SN - 0021-9738
IS - 4
ER -