Adipose-derived stem cells and the stromal vascular fraction in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration

Mari Shimizu, Hajime Matsumine, Hironobu Osaki, Yoshifumi Ueta, Satoshi Tsunoda, Wataru Kamei, Kazuki Hashimoto, Yosuke Niimi, Yorikatsu Watanabe, Mariko Miyata, Hiroyuki Sakurai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Adipose-derived stem cells (ADSCs) and the stromal vascular fraction (SVF) promote nerve regeneration. Biodegradable nerve conduits are used to treat peripheral nerve injuries, but their efficiencies are lower than those of autologous nerve grafts. This study developed biodegradable nerve conduits containing ADSCs and SVF and evaluated their facial nerve regenerating abilities in a rat model with a 7-mm nerve defect. SVF and ADSCs were individually poured into nerve conduits with polyglycolic acid-type I collagen as a scaffold (ADSCs and SVF groups). The conduits were grafted on to the nerve defects. As the control, the defect was bridged with polyglycolic acid-collagen nerve conduits without cells. At 13 weeks, after transplantation, the regenerated nerves were evaluated physiologically and histologically. The compound muscle action potential of the SVF group was significantly higher in amplitude than that of the control group. Electron microscopy showed that the axon diameter of the SVF group was the largest, followed by the ADSC group and control group with significant differences among them. The SVF group had the largest fiber diameter, followed by the ADSC group and control group with significant differences among them. The ADSC group had the highest myelin thickness, followed by the SVF group and control group with significant differences among them. Identical excellent promoting effects on nerve regeneration were observed in both the ADSC and SVF groups. Using SVF in conduits was more practical than using ADSCs because only the enzymatic process was required to prepare SVF, indicating that SVF could be more suitable to induce nerve regeneration.

Original languageEnglish (US)
Pages (from-to)446-455
Number of pages10
JournalWound Repair and Regeneration
Volume26
Issue number6
DOIs
StatePublished - Nov 1 2018
Externally publishedYes

Fingerprint

Polyglycolic Acid
Nerve Regeneration
Facial Nerve
Blood Vessels
Collagen
Stem Cells
Control Groups
Peripheral Nerve Injuries
Myelin Sheath
Collagen Type I
Action Potentials
Axons
Electron Microscopy

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Adipose-derived stem cells and the stromal vascular fraction in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration. / Shimizu, Mari; Matsumine, Hajime; Osaki, Hironobu; Ueta, Yoshifumi; Tsunoda, Satoshi; Kamei, Wataru; Hashimoto, Kazuki; Niimi, Yosuke; Watanabe, Yorikatsu; Miyata, Mariko; Sakurai, Hiroyuki.

In: Wound Repair and Regeneration, Vol. 26, No. 6, 01.11.2018, p. 446-455.

Research output: Contribution to journalArticle

Shimizu, M, Matsumine, H, Osaki, H, Ueta, Y, Tsunoda, S, Kamei, W, Hashimoto, K, Niimi, Y, Watanabe, Y, Miyata, M & Sakurai, H 2018, 'Adipose-derived stem cells and the stromal vascular fraction in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration', Wound Repair and Regeneration, vol. 26, no. 6, pp. 446-455. https://doi.org/10.1111/wrr.12665
Shimizu, Mari ; Matsumine, Hajime ; Osaki, Hironobu ; Ueta, Yoshifumi ; Tsunoda, Satoshi ; Kamei, Wataru ; Hashimoto, Kazuki ; Niimi, Yosuke ; Watanabe, Yorikatsu ; Miyata, Mariko ; Sakurai, Hiroyuki. / Adipose-derived stem cells and the stromal vascular fraction in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration. In: Wound Repair and Regeneration. 2018 ; Vol. 26, No. 6. pp. 446-455.
@article{898e6793a0f94610a39cf9284e994c16,
title = "Adipose-derived stem cells and the stromal vascular fraction in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration",
abstract = "Adipose-derived stem cells (ADSCs) and the stromal vascular fraction (SVF) promote nerve regeneration. Biodegradable nerve conduits are used to treat peripheral nerve injuries, but their efficiencies are lower than those of autologous nerve grafts. This study developed biodegradable nerve conduits containing ADSCs and SVF and evaluated their facial nerve regenerating abilities in a rat model with a 7-mm nerve defect. SVF and ADSCs were individually poured into nerve conduits with polyglycolic acid-type I collagen as a scaffold (ADSCs and SVF groups). The conduits were grafted on to the nerve defects. As the control, the defect was bridged with polyglycolic acid-collagen nerve conduits without cells. At 13 weeks, after transplantation, the regenerated nerves were evaluated physiologically and histologically. The compound muscle action potential of the SVF group was significantly higher in amplitude than that of the control group. Electron microscopy showed that the axon diameter of the SVF group was the largest, followed by the ADSC group and control group with significant differences among them. The SVF group had the largest fiber diameter, followed by the ADSC group and control group with significant differences among them. The ADSC group had the highest myelin thickness, followed by the SVF group and control group with significant differences among them. Identical excellent promoting effects on nerve regeneration were observed in both the ADSC and SVF groups. Using SVF in conduits was more practical than using ADSCs because only the enzymatic process was required to prepare SVF, indicating that SVF could be more suitable to induce nerve regeneration.",
author = "Mari Shimizu and Hajime Matsumine and Hironobu Osaki and Yoshifumi Ueta and Satoshi Tsunoda and Wataru Kamei and Kazuki Hashimoto and Yosuke Niimi and Yorikatsu Watanabe and Mariko Miyata and Hiroyuki Sakurai",
year = "2018",
month = "11",
day = "1",
doi = "10.1111/wrr.12665",
language = "English (US)",
volume = "26",
pages = "446--455",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Adipose-derived stem cells and the stromal vascular fraction in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration

AU - Shimizu, Mari

AU - Matsumine, Hajime

AU - Osaki, Hironobu

AU - Ueta, Yoshifumi

AU - Tsunoda, Satoshi

AU - Kamei, Wataru

AU - Hashimoto, Kazuki

AU - Niimi, Yosuke

AU - Watanabe, Yorikatsu

AU - Miyata, Mariko

AU - Sakurai, Hiroyuki

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Adipose-derived stem cells (ADSCs) and the stromal vascular fraction (SVF) promote nerve regeneration. Biodegradable nerve conduits are used to treat peripheral nerve injuries, but their efficiencies are lower than those of autologous nerve grafts. This study developed biodegradable nerve conduits containing ADSCs and SVF and evaluated their facial nerve regenerating abilities in a rat model with a 7-mm nerve defect. SVF and ADSCs were individually poured into nerve conduits with polyglycolic acid-type I collagen as a scaffold (ADSCs and SVF groups). The conduits were grafted on to the nerve defects. As the control, the defect was bridged with polyglycolic acid-collagen nerve conduits without cells. At 13 weeks, after transplantation, the regenerated nerves were evaluated physiologically and histologically. The compound muscle action potential of the SVF group was significantly higher in amplitude than that of the control group. Electron microscopy showed that the axon diameter of the SVF group was the largest, followed by the ADSC group and control group with significant differences among them. The SVF group had the largest fiber diameter, followed by the ADSC group and control group with significant differences among them. The ADSC group had the highest myelin thickness, followed by the SVF group and control group with significant differences among them. Identical excellent promoting effects on nerve regeneration were observed in both the ADSC and SVF groups. Using SVF in conduits was more practical than using ADSCs because only the enzymatic process was required to prepare SVF, indicating that SVF could be more suitable to induce nerve regeneration.

AB - Adipose-derived stem cells (ADSCs) and the stromal vascular fraction (SVF) promote nerve regeneration. Biodegradable nerve conduits are used to treat peripheral nerve injuries, but their efficiencies are lower than those of autologous nerve grafts. This study developed biodegradable nerve conduits containing ADSCs and SVF and evaluated their facial nerve regenerating abilities in a rat model with a 7-mm nerve defect. SVF and ADSCs were individually poured into nerve conduits with polyglycolic acid-type I collagen as a scaffold (ADSCs and SVF groups). The conduits were grafted on to the nerve defects. As the control, the defect was bridged with polyglycolic acid-collagen nerve conduits without cells. At 13 weeks, after transplantation, the regenerated nerves were evaluated physiologically and histologically. The compound muscle action potential of the SVF group was significantly higher in amplitude than that of the control group. Electron microscopy showed that the axon diameter of the SVF group was the largest, followed by the ADSC group and control group with significant differences among them. The SVF group had the largest fiber diameter, followed by the ADSC group and control group with significant differences among them. The ADSC group had the highest myelin thickness, followed by the SVF group and control group with significant differences among them. Identical excellent promoting effects on nerve regeneration were observed in both the ADSC and SVF groups. Using SVF in conduits was more practical than using ADSCs because only the enzymatic process was required to prepare SVF, indicating that SVF could be more suitable to induce nerve regeneration.

UR - http://www.scopus.com/inward/record.url?scp=85055698144&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055698144&partnerID=8YFLogxK

U2 - 10.1111/wrr.12665

DO - 10.1111/wrr.12665

M3 - Article

C2 - 30118577

AN - SCOPUS:85055698144

VL - 26

SP - 446

EP - 455

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 6

ER -