Adipose tissue uncoupling protein 1 levels and function are increased in a mouse model of developmental obesity induced by maternal exposure to high-fat diet

E. Bytautiene Prewit, Craig Porter, M. La Rosa, N. Bhattarai, H. Yin, P. Gamble, T. Kechichian, L. S. Sidossis

Research output: Contribution to journalArticle

Abstract

With brown adipose tissue (BAT) becoming a possible therapeutic target to counteract obesity, the prenatal environment could represent a critical window to modify BAT function and browning of white AT. We investigated if levels of uncoupling protein 1 (UCP1) and UCP1-mediated thermogenesis are altered in offspring exposed to prenatal obesity. Female CD-1 mice were fed a high-fat (HF) or standard-fat (SF) diet for 3 months before breeding. After weaning, all pups were placed on SF. UCP1 mRNA and protein levels were quantified using quantitative real-time PCR and Western blot analysis, respectively, in brown (BAT), subcutaneous (SAT) and visceral (VAT) adipose tissues at 6 months of age. Total and UCP1-dependent mitochondrial respiration were determined by high-resolution respirometry. A Student's t-test and Mann-Whitney test were used (significance: P<0.05). UCP1 mRNA levels were not different between the HF and SF offspring. UCP1 protein levels, total mitochondrial respiration and UCP1-dependent respiration were significantly higher in BAT from HF males (P=0.02, P=0.04, P=0.005, respectively) and females (P=0.01, P=0.04, P=0.02, respectively). In SAT, the UCP1 protein was significantly lower in HF females (P=0.03), and the UCP1-dependent thermogenesis was significantly lower from HF males (P=0.04). In VAT, UCP1 protein levels and UCP1-dependent respiration were significantly lower only in HF females (P=0.03, P=0.04, respectively). There were no differences in total respiration in SAT and VAT. Prenatal exposure to maternal obesity leads to significant increases in UCP1 levels and function in BAT in offspring with little impact on UCP1 levels and function in SAT and VAT.

Original languageEnglish (US)
Pages (from-to)401-408
Number of pages8
JournalJournal of Developmental Origins of Health and Disease
Volume9
Issue number4
DOIs
StatePublished - Aug 1 2018

Fingerprint

Maternal Exposure
High Fat Diet
Adipose Tissue
Obesity
Fats
Brown Adipose Tissue
Respiration
Thermogenesis
Uncoupling Protein 1
Proteins
Messenger RNA
Intra-Abdominal Fat
Weaning
Breeding
Real-Time Polymerase Chain Reaction

Keywords

  • brown adipose tissue
  • developmental programming
  • obesity
  • UCP1

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

Adipose tissue uncoupling protein 1 levels and function are increased in a mouse model of developmental obesity induced by maternal exposure to high-fat diet. / Bytautiene Prewit, E.; Porter, Craig; La Rosa, M.; Bhattarai, N.; Yin, H.; Gamble, P.; Kechichian, T.; Sidossis, L. S.

In: Journal of Developmental Origins of Health and Disease, Vol. 9, No. 4, 01.08.2018, p. 401-408.

Research output: Contribution to journalArticle

Bytautiene Prewit, E. ; Porter, Craig ; La Rosa, M. ; Bhattarai, N. ; Yin, H. ; Gamble, P. ; Kechichian, T. ; Sidossis, L. S. / Adipose tissue uncoupling protein 1 levels and function are increased in a mouse model of developmental obesity induced by maternal exposure to high-fat diet. In: Journal of Developmental Origins of Health and Disease. 2018 ; Vol. 9, No. 4. pp. 401-408.
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