Administration of a peroxynitrite decomposition catalyst into the bronchial artery attenuates pulmonary dysfunction after smoke inhalation and burn injury in sheep

Atsumori Hamahata, Perenlei Enkhbaatar, Matthias Lange, Takashi Yamaki, Hiroaki Nakazawa, Motohiro Nozaki, Hiroyuki Sakurai, Lillian D. Traber, Daniel L. Traber

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Reactive nitrogen species such as peroxynitrite play a significant role in burn and smoke inhalation injury. The bronchial circulation increases more than 10-fold in response to this combination injury. We hypothesized that direct delivery of low-dose WW-85, a peroxynitrite decomposition catalyst, into the bronchial artery would attenuate burn-and smoke inhalation-induced acute lung injury. In adult female sheep (n = 17), the bronchial artery was cannulated in preparation surgery. After a 5-to 7-day recovery period, sheep were subjected to a burn (40% total body surface area, third degree) and inhalation injury (48 breaths of cotton smoke, <40°C). The animals were divided into three groups following the injury: (i) WW-85 group: 1 h after injury, WW-85 (0.002 mg/kg per hour) was continuously infused into the bronchial artery, n = 5; (ii) control group: 1 h after injury, an equivalent amount of saline was injected into the bronchial artery, n = 6; (iii) sham group: no injury, no treatment, same operation and anesthesia, n = 6. All animals were mechanically ventilated and fluid resuscitated equally. In the control group, the injury induced a severe deterioration of pulmonary oxygenation and shunting and an increase in pulmonary microvascular permeability toward sham. The injury was further associated with an increase in reactive nitrogen species in lung tissues of the control group. All these alterations were significantly attenuated in the WW-85 group. We demonstrated that a low dosage of WW-85 directly administered into the bronchial artery attenuated pulmonary dysfunction to the same extent as higher systemically administered doses in previous experiments. Our data strongly suggest that local airway production of peroxynitrite contributes to pulmonary dysfunction following smoke inhalation and burn injury.

Original languageEnglish (US)
Pages (from-to)543-548
Number of pages6
JournalShock
Volume38
Issue number5
DOIs
StatePublished - Nov 2012

Fingerprint

Inhalation Burns
Smoke Inhalation Injury
Bronchial Arteries
Peroxynitrous Acid
Sheep
Lung
Wounds and Injuries
Reactive Nitrogen Species
Smoke
Control Groups
Acute Lung Injury
Body Surface Area
Capillary Permeability
Inhalation
Anesthesia

Keywords

  • bronchial artery
  • Burn
  • peroxynitrite
  • peroxynitrite decomposition catalyst
  • smoke inhalation
  • WW-85

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Administration of a peroxynitrite decomposition catalyst into the bronchial artery attenuates pulmonary dysfunction after smoke inhalation and burn injury in sheep. / Hamahata, Atsumori; Enkhbaatar, Perenlei; Lange, Matthias; Yamaki, Takashi; Nakazawa, Hiroaki; Nozaki, Motohiro; Sakurai, Hiroyuki; Traber, Lillian D.; Traber, Daniel L.

In: Shock, Vol. 38, No. 5, 11.2012, p. 543-548.

Research output: Contribution to journalArticle

Hamahata, Atsumori ; Enkhbaatar, Perenlei ; Lange, Matthias ; Yamaki, Takashi ; Nakazawa, Hiroaki ; Nozaki, Motohiro ; Sakurai, Hiroyuki ; Traber, Lillian D. ; Traber, Daniel L. / Administration of a peroxynitrite decomposition catalyst into the bronchial artery attenuates pulmonary dysfunction after smoke inhalation and burn injury in sheep. In: Shock. 2012 ; Vol. 38, No. 5. pp. 543-548.
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