Administration of antenatal glucocorticoids and postnatal surfactant ameliorates respiratory distress syndrome associated neonatal lethality in Erk3?/? mouse pups

Milenka Cuevas Guaman, Elena Sbrana, Cynthia Shope, Lori Showalter, Min Hu, Sylvain Meloche, Kjersti Aagaard

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background:Respiratory distress syndrome (RDS) persists as a prevalent cause of infant morbidity and mortality. We have previously demonstrated that deletion of Erk3 results in pulmonary immaturity and neonatal lethality. Using RNA sequencing, we identified corticotrophin releasing hormone (CRH) and surfactant protein B (SFTPB) as potential molecular mediators of Erk3-dependent lung maturation. In this study, we characterized the impact of antenatal glucocorticoids and postnatal surfactant on neonatal survival of Erk3 null mice.Methods:In a double crossover design, we administered dexamethasone (dex) or saline to pregnant dams during the saccular stage of lung development, followed by postnatal surfactant or saline via inhalation intubation. Survival was recorded, and detailed lung histological analysis and staining for CRH and SFTPB protein expression were performed.Results:Without treatment, Erk3 null pups die within 6 h of birth with reduced aerated space, impaired thinning of the alveolar septa, and abundant glycogen stores, as described in human RDS. The administration of dex and surfactant improved RDS-Associated lethality of Erk3 -/- pups and partially restored functional fetal lung maturation by accelerating the downregulation of pulmonary CRH and partially rescuing the production of SFTPB.Conclusion:These findings emphasize that Erk3 is integral to terminal differentiation of type II cells, SFTPB production, and fetal pulmonary maturity.

Original languageEnglish (US)
Pages (from-to)24-32
Number of pages9
JournalPediatric Research
Volume76
Issue number1
DOIs
StatePublished - 2014

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Newborn Respiratory Distress Syndrome
Surface-Active Agents
Glucocorticoids
Lung
Corticotropin-Releasing Hormone
Dexamethasone
RNA Sequence Analysis
Survival
Infant Mortality
Glycogen
Intubation
Cross-Over Studies
Inhalation
Cell Differentiation
Down-Regulation
Parturition
Staining and Labeling
Morbidity
IgA receptor

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Administration of antenatal glucocorticoids and postnatal surfactant ameliorates respiratory distress syndrome associated neonatal lethality in Erk3?/? mouse pups. / Cuevas Guaman, Milenka; Sbrana, Elena; Shope, Cynthia; Showalter, Lori; Hu, Min; Meloche, Sylvain; Aagaard, Kjersti.

In: Pediatric Research, Vol. 76, No. 1, 2014, p. 24-32.

Research output: Contribution to journalArticle

Cuevas Guaman, Milenka ; Sbrana, Elena ; Shope, Cynthia ; Showalter, Lori ; Hu, Min ; Meloche, Sylvain ; Aagaard, Kjersti. / Administration of antenatal glucocorticoids and postnatal surfactant ameliorates respiratory distress syndrome associated neonatal lethality in Erk3?/? mouse pups. In: Pediatric Research. 2014 ; Vol. 76, No. 1. pp. 24-32.
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AU - Hu, Min

AU - Meloche, Sylvain

AU - Aagaard, Kjersti

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AB - Background:Respiratory distress syndrome (RDS) persists as a prevalent cause of infant morbidity and mortality. We have previously demonstrated that deletion of Erk3 results in pulmonary immaturity and neonatal lethality. Using RNA sequencing, we identified corticotrophin releasing hormone (CRH) and surfactant protein B (SFTPB) as potential molecular mediators of Erk3-dependent lung maturation. In this study, we characterized the impact of antenatal glucocorticoids and postnatal surfactant on neonatal survival of Erk3 null mice.Methods:In a double crossover design, we administered dexamethasone (dex) or saline to pregnant dams during the saccular stage of lung development, followed by postnatal surfactant or saline via inhalation intubation. Survival was recorded, and detailed lung histological analysis and staining for CRH and SFTPB protein expression were performed.Results:Without treatment, Erk3 null pups die within 6 h of birth with reduced aerated space, impaired thinning of the alveolar septa, and abundant glycogen stores, as described in human RDS. The administration of dex and surfactant improved RDS-Associated lethality of Erk3 -/- pups and partially restored functional fetal lung maturation by accelerating the downregulation of pulmonary CRH and partially rescuing the production of SFTPB.Conclusion:These findings emphasize that Erk3 is integral to terminal differentiation of type II cells, SFTPB production, and fetal pulmonary maturity.

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