TY - JOUR
T1 - Adult patients are more catabolic than children during acute phase after burn injury
T2 - A retrospective analysis on muscle protein kinetics
AU - Tuvdendorj, Demidmaa
AU - Chinkes, David L.
AU - Zhang, Xiao Jun
AU - Ferrando, Arny A.
AU - Elijah, Itoro E.
AU - Mlcak, Ronald P.
AU - Finnerty, Celeste C.
AU - Wolfe, Robert R.
AU - Herndon, David N.
N1 - Funding Information:
Acknowledgments The authors wish to thank the study volunteers and their families for their patience and dedication, and the clinical and research staff of Shriners Hospital for Children and Blocker Adult Burn Unit, UTMB, Galveston, TX for their help with conducting the clinical portion of the study; Tara Cocke, Christopher Danesi, Ann Hightower, and Cindy Locklin for excellent technical performance; Guy Jones and Jariwala Guarang for performing GC–MS analyses, and Steve Schuenke for his excellent technical assistance in the preparation of this manuscript. This work was supported, in part, by NIH Grants R01-GM56687, P50-GM60338, R01-GM57295, and T32-GM08256 as well as the Shriners Mass Spectrometry Core Grant 84090. CCF is an ITS Career Development Scholar supported, in part, by NIH KL2RR029875 and NIH UL1RR029876 (ARB).
PY - 2011/8
Y1 - 2011/8
N2 - Purpose: This study was performed to determine if there is an age-related specificity in the response of muscle protein metabolism to severe burn injury during acute hospitalization. This is a retrospective analysis of previously published data. Methods: Nineteen adult and 58 pediatric burn-injured patients (age 43.3 ± 14.3 vs. 7.2 ± 5.3 years, adult vs. children) participated in stable isotope [ring- 2H 5]phenylalanine (Phe) infusion studies. Femoral arterial and venous blood samples and muscle biopsy samples were collected throughout the study. Data are presented as means ± standard deviation (SD). A p value less than 0.05 was considered statistically significant. Results: Muscle net protein balance (NB) was higher in children (adult vs. children, -43 ± 61 vs. 8 ± 68 nmol Phe/min/100 ml leg volume, p < 0.05). Muscle protein fractional synthesis rate (FSR) was higher in children (adult vs. children, 0.11 ± 0.05 vs. 0.16 ± 0.10 %/h, p < 0.05). Leg muscle protein breakdown was not different between the groups (adult vs. children, 179 ± 115 vs. 184 ± 124 nmol Phe/min/100 ml leg volume, p > 0.05); synthesis rate was 134 ± 96 and 192 ± 128 nmol Phe/min/100 ml leg volume in adults and children, respectively (p = 0.07). Age significantly correlated with muscle protein NB (p = 0.01) and FSR (p = 0.02); but not with breakdown (p = 0.67) and synthesis (p = 0.07) rates measured by using a three-pool model. Conclusion: In burn injury, the muscle protein breakdown may be affected to the same extent in adults and children, whereas synthesis may have age-related specificities, resulting in a better but still low NB in children.
AB - Purpose: This study was performed to determine if there is an age-related specificity in the response of muscle protein metabolism to severe burn injury during acute hospitalization. This is a retrospective analysis of previously published data. Methods: Nineteen adult and 58 pediatric burn-injured patients (age 43.3 ± 14.3 vs. 7.2 ± 5.3 years, adult vs. children) participated in stable isotope [ring- 2H 5]phenylalanine (Phe) infusion studies. Femoral arterial and venous blood samples and muscle biopsy samples were collected throughout the study. Data are presented as means ± standard deviation (SD). A p value less than 0.05 was considered statistically significant. Results: Muscle net protein balance (NB) was higher in children (adult vs. children, -43 ± 61 vs. 8 ± 68 nmol Phe/min/100 ml leg volume, p < 0.05). Muscle protein fractional synthesis rate (FSR) was higher in children (adult vs. children, 0.11 ± 0.05 vs. 0.16 ± 0.10 %/h, p < 0.05). Leg muscle protein breakdown was not different between the groups (adult vs. children, 179 ± 115 vs. 184 ± 124 nmol Phe/min/100 ml leg volume, p > 0.05); synthesis rate was 134 ± 96 and 192 ± 128 nmol Phe/min/100 ml leg volume in adults and children, respectively (p = 0.07). Age significantly correlated with muscle protein NB (p = 0.01) and FSR (p = 0.02); but not with breakdown (p = 0.67) and synthesis (p = 0.07) rates measured by using a three-pool model. Conclusion: In burn injury, the muscle protein breakdown may be affected to the same extent in adults and children, whereas synthesis may have age-related specificities, resulting in a better but still low NB in children.
KW - Age
KW - Burn
KW - Muscle protein metabolism
UR - https://www.scopus.com/pages/publications/79961031571
UR - https://www.scopus.com/pages/publications/79961031571#tab=citedBy
U2 - 10.1007/s00134-011-2223-3
DO - 10.1007/s00134-011-2223-3
M3 - Article
C2 - 21647721
AN - SCOPUS:79961031571
SN - 0342-4642
VL - 37
SP - 1317
EP - 1322
JO - Intensive care medicine
JF - Intensive care medicine
IS - 8
ER -