Adverse effects of methylphenidate on the reproductive axis of adolescent female rats

Shilla Chatterjee-Chakrabarty, Brian T. Miller, Thomas J. Collins, Manubai Nagamani

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: To examine the effects of chronic methylphenidate use on the reproductive axis of adolescent female rats. Design: Controlled prospective study. Setting: University research laboratory. Animal(s): Twenty prepubertal female Sprague Dawley rats. Intervention(s): Subcutaneous implantation of drug-filled Alzet minipumps (Durect Corporation, Cupertino, CA) for infusing methylphenidate (450 μg/d, treated) or physiological saline (control) for 4 weeks. Estrous cyclicity was checked from 3 weeks of pump implantation till the termination of the experiments. Animals were killed after 4 weeks of treatment. Main Outcome Measure(s): Estrous cyclicity, pituitary and peripheral FSH and LH, serum estrogen and progesterone, ovarian histology, and immunocytochemistry for localizing growth differentiation factor-9 and activin receptors-I. Result(s): Compared with the control group, the treated animals exhibited the following: [1] poor vaginal opening and erratic estrous cyclicity; [2] undeveloped, disrupted, or prematurely luteinized ovarian follicles; [3] absence of growth differentiation factor-9 and of activin receptors I and IB in the oocyte; and [4] high levels of LH in the pituitary. Conclusion(s): Chronic methylphenidate administration during adolescence perturbs pubertal onset, adversely affects maturation of the female reproductive axis by retarding pituitary LH release, and adversely affects ovarian folliculogenesis. These novel findings may have significant clinical implications in evaluating the effects of methylphenidate abuse on adolescent health.

Original languageEnglish (US)
Pages (from-to)1131-1138
Number of pages8
JournalFertility and Sterility
Volume84
Issue numberSUPPL. 2
DOIs
StatePublished - Oct 2005

Fingerprint

Methylphenidate
Growth Differentiation Factor 9
Periodicity
Activin Receptors
Ovarian Follicle
Oocytes
Progesterone
Sprague Dawley Rats
Histology
Estrogens
Immunohistochemistry
Outcome Assessment (Health Care)
Prospective Studies
Control Groups
Serum
Research
Pharmaceutical Preparations
Therapeutics

Keywords

  • Adolescent female rats
  • Methylphenidate
  • Reproductive axis

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Chatterjee-Chakrabarty, S., Miller, B. T., Collins, T. J., & Nagamani, M. (2005). Adverse effects of methylphenidate on the reproductive axis of adolescent female rats. Fertility and Sterility, 84(SUPPL. 2), 1131-1138. https://doi.org/10.1016/j.fertnstert.2005.03.071

Adverse effects of methylphenidate on the reproductive axis of adolescent female rats. / Chatterjee-Chakrabarty, Shilla; Miller, Brian T.; Collins, Thomas J.; Nagamani, Manubai.

In: Fertility and Sterility, Vol. 84, No. SUPPL. 2, 10.2005, p. 1131-1138.

Research output: Contribution to journalArticle

Chatterjee-Chakrabarty, S, Miller, BT, Collins, TJ & Nagamani, M 2005, 'Adverse effects of methylphenidate on the reproductive axis of adolescent female rats', Fertility and Sterility, vol. 84, no. SUPPL. 2, pp. 1131-1138. https://doi.org/10.1016/j.fertnstert.2005.03.071
Chatterjee-Chakrabarty, Shilla ; Miller, Brian T. ; Collins, Thomas J. ; Nagamani, Manubai. / Adverse effects of methylphenidate on the reproductive axis of adolescent female rats. In: Fertility and Sterility. 2005 ; Vol. 84, No. SUPPL. 2. pp. 1131-1138.
@article{10e8b0ece8f6496b9cdaa51a759fab73,
title = "Adverse effects of methylphenidate on the reproductive axis of adolescent female rats",
abstract = "Objective: To examine the effects of chronic methylphenidate use on the reproductive axis of adolescent female rats. Design: Controlled prospective study. Setting: University research laboratory. Animal(s): Twenty prepubertal female Sprague Dawley rats. Intervention(s): Subcutaneous implantation of drug-filled Alzet minipumps (Durect Corporation, Cupertino, CA) for infusing methylphenidate (450 μg/d, treated) or physiological saline (control) for 4 weeks. Estrous cyclicity was checked from 3 weeks of pump implantation till the termination of the experiments. Animals were killed after 4 weeks of treatment. Main Outcome Measure(s): Estrous cyclicity, pituitary and peripheral FSH and LH, serum estrogen and progesterone, ovarian histology, and immunocytochemistry for localizing growth differentiation factor-9 and activin receptors-I. Result(s): Compared with the control group, the treated animals exhibited the following: [1] poor vaginal opening and erratic estrous cyclicity; [2] undeveloped, disrupted, or prematurely luteinized ovarian follicles; [3] absence of growth differentiation factor-9 and of activin receptors I and IB in the oocyte; and [4] high levels of LH in the pituitary. Conclusion(s): Chronic methylphenidate administration during adolescence perturbs pubertal onset, adversely affects maturation of the female reproductive axis by retarding pituitary LH release, and adversely affects ovarian folliculogenesis. These novel findings may have significant clinical implications in evaluating the effects of methylphenidate abuse on adolescent health.",
keywords = "Adolescent female rats, Methylphenidate, Reproductive axis",
author = "Shilla Chatterjee-Chakrabarty and Miller, {Brian T.} and Collins, {Thomas J.} and Manubai Nagamani",
year = "2005",
month = "10",
doi = "10.1016/j.fertnstert.2005.03.071",
language = "English (US)",
volume = "84",
pages = "1131--1138",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "SUPPL. 2",

}

TY - JOUR

T1 - Adverse effects of methylphenidate on the reproductive axis of adolescent female rats

AU - Chatterjee-Chakrabarty, Shilla

AU - Miller, Brian T.

AU - Collins, Thomas J.

AU - Nagamani, Manubai

PY - 2005/10

Y1 - 2005/10

N2 - Objective: To examine the effects of chronic methylphenidate use on the reproductive axis of adolescent female rats. Design: Controlled prospective study. Setting: University research laboratory. Animal(s): Twenty prepubertal female Sprague Dawley rats. Intervention(s): Subcutaneous implantation of drug-filled Alzet minipumps (Durect Corporation, Cupertino, CA) for infusing methylphenidate (450 μg/d, treated) or physiological saline (control) for 4 weeks. Estrous cyclicity was checked from 3 weeks of pump implantation till the termination of the experiments. Animals were killed after 4 weeks of treatment. Main Outcome Measure(s): Estrous cyclicity, pituitary and peripheral FSH and LH, serum estrogen and progesterone, ovarian histology, and immunocytochemistry for localizing growth differentiation factor-9 and activin receptors-I. Result(s): Compared with the control group, the treated animals exhibited the following: [1] poor vaginal opening and erratic estrous cyclicity; [2] undeveloped, disrupted, or prematurely luteinized ovarian follicles; [3] absence of growth differentiation factor-9 and of activin receptors I and IB in the oocyte; and [4] high levels of LH in the pituitary. Conclusion(s): Chronic methylphenidate administration during adolescence perturbs pubertal onset, adversely affects maturation of the female reproductive axis by retarding pituitary LH release, and adversely affects ovarian folliculogenesis. These novel findings may have significant clinical implications in evaluating the effects of methylphenidate abuse on adolescent health.

AB - Objective: To examine the effects of chronic methylphenidate use on the reproductive axis of adolescent female rats. Design: Controlled prospective study. Setting: University research laboratory. Animal(s): Twenty prepubertal female Sprague Dawley rats. Intervention(s): Subcutaneous implantation of drug-filled Alzet minipumps (Durect Corporation, Cupertino, CA) for infusing methylphenidate (450 μg/d, treated) or physiological saline (control) for 4 weeks. Estrous cyclicity was checked from 3 weeks of pump implantation till the termination of the experiments. Animals were killed after 4 weeks of treatment. Main Outcome Measure(s): Estrous cyclicity, pituitary and peripheral FSH and LH, serum estrogen and progesterone, ovarian histology, and immunocytochemistry for localizing growth differentiation factor-9 and activin receptors-I. Result(s): Compared with the control group, the treated animals exhibited the following: [1] poor vaginal opening and erratic estrous cyclicity; [2] undeveloped, disrupted, or prematurely luteinized ovarian follicles; [3] absence of growth differentiation factor-9 and of activin receptors I and IB in the oocyte; and [4] high levels of LH in the pituitary. Conclusion(s): Chronic methylphenidate administration during adolescence perturbs pubertal onset, adversely affects maturation of the female reproductive axis by retarding pituitary LH release, and adversely affects ovarian folliculogenesis. These novel findings may have significant clinical implications in evaluating the effects of methylphenidate abuse on adolescent health.

KW - Adolescent female rats

KW - Methylphenidate

KW - Reproductive axis

UR - http://www.scopus.com/inward/record.url?scp=25844485569&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25844485569&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2005.03.071

DO - 10.1016/j.fertnstert.2005.03.071

M3 - Article

C2 - 16210004

AN - SCOPUS:25844485569

VL - 84

SP - 1131

EP - 1138

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - SUPPL. 2

ER -