Aedes mosquitoes acquire and transmit Zika virus by breeding in contaminated aquatic environments

Senyan Du; Yang Liu; Jianying Liu; Jie Zhao; Clara Champagne; Liangqin Tong; Renli Zhang; Fuchun Zhang; Cheng Feng Qin; Ping Ma; Chun Hong Chen; Guodong Liang; Qiyong Liu; Pei Yong Shi; Bernard Cazelles; Penghua Wang; Huaiyu Tian; Gong Cheng

doi:10.1038/s41467-019-09256-0

Aedes mosquitoes acquire and transmit Zika virus by breeding in contaminated aquatic environments

Senyan Du, Yang Liu, Jianying Liu, Jie Zhao, Clara Champagne, Liangqin Tong, Renli Zhang, Fuchun Zhang, Cheng Feng Qin, Ping Ma, Chun Hong Chen, Guodong Liang, Qiyong Liu, Pei Yong Shi, Bernard Cazelles, Penghua Wang, Huaiyu Tian, Gong Cheng

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Zika virus (ZIKV) is a mosquito-borne flavivirus that predominantly circulates between humans and Aedes mosquitoes. Clinical studies have shown that Zika viruria in patients persists for an extended period, and results in infectious virions being excreted. Here, we demonstrate that Aedes mosquitoes are permissive to ZIKV infection when breeding in urine or sewage containing low concentrations of ZIKV. Mosquito larvae and pupae, including from field Aedes aegypti can acquire ZIKV from contaminated aquatic systems, resulting in ZIKV infection of adult females. Adult mosquitoes can transmit infectious virions to susceptible type I/II interferon receptor-deficient (ifnagr-/-) C57BL/6 (AG6) mice. Furthermore, ZIKV viruria from infected AG6 mice can causes mosquito infection during the aquatic life stages. Our studies suggest that infectious urine could be a natural ZIKV source, which is potentially transmissible to mosquitoes when breeding in an aquatic environment.

Original language	English (US)
Article number	1324
Journal	Nature communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-09256-0
State	Published - Dec 1 2019

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
General
Physics and Astronomy(all)

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Du, S., Liu, Y., Liu, J., Zhao, J., Champagne, C., Tong, L., Zhang, R., Zhang, F., Qin, C. F., Ma, P., Chen, C. H., Liang, G., Liu, Q., Shi, P. Y., Cazelles, B., Wang, P., Tian, H., & Cheng, G. (2019). Aedes mosquitoes acquire and transmit Zika virus by breeding in contaminated aquatic environments. Nature communications, 10(1), [1324]. https://doi.org/10.1038/s41467-019-09256-0

@article{3b88c2bf9d8e4f82b3061d935a0d5954,

title = " Aedes mosquitoes acquire and transmit Zika virus by breeding in contaminated aquatic environments",

abstract = "Zika virus (ZIKV) is a mosquito-borne flavivirus that predominantly circulates between humans and Aedes mosquitoes. Clinical studies have shown that Zika viruria in patients persists for an extended period, and results in infectious virions being excreted. Here, we demonstrate that Aedes mosquitoes are permissive to ZIKV infection when breeding in urine or sewage containing low concentrations of ZIKV. Mosquito larvae and pupae, including from field Aedes aegypti can acquire ZIKV from contaminated aquatic systems, resulting in ZIKV infection of adult females. Adult mosquitoes can transmit infectious virions to susceptible type I/II interferon receptor-deficient (ifnagr-/-) C57BL/6 (AG6) mice. Furthermore, ZIKV viruria from infected AG6 mice can causes mosquito infection during the aquatic life stages. Our studies suggest that infectious urine could be a natural ZIKV source, which is potentially transmissible to mosquitoes when breeding in an aquatic environment.",

author = "Senyan Du and Yang Liu and Jianying Liu and Jie Zhao and Clara Champagne and Liangqin Tong and Renli Zhang and Fuchun Zhang and Qin, {Cheng Feng} and Ping Ma and Chen, {Chun Hong} and Guodong Liang and Qiyong Liu and Shi, {Pei Yong} and Bernard Cazelles and Penghua Wang and Huaiyu Tian and Gong Cheng",

note = "Funding Information: This work was funded by grants from the National Key Research and Development Plan of China (2018ZX09711003-004-003, 2018YFA0507202, 2016YFD0500400, 2017ZX10305501-003), the Natural Science Foundation of China (31825001, 81730063, 81571975, 81673234), the Shenzhen San-Ming Project for prevention and research on vector-borne diseases (SZSM201611064), and the Municipal Healthcare Joint-Innovation Major Project of Guangzhou (201704020229). P.W. is supported by a National Institutes of Health award R01AI132526. We thank that Professor Scott Weaver from the UTMB provided critical suggestions for the manuscript. G.C. is a Newton Advanced Fellow awarded by the Academy of Medical Sciences and the Newton Fund. We also thank the core facilities of the Center for Life Sciences and Center of Biomedical Analysis for technical assistance (Tsinghua University). Publisher Copyright: {\textcopyright} 2019, The Author(s).",

year = "2019",

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doi = "10.1038/s41467-019-09256-0",

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T1 - Aedes mosquitoes acquire and transmit Zika virus by breeding in contaminated aquatic environments

AU - Du, Senyan

AU - Liu, Yang

AU - Liu, Jianying

AU - Zhao, Jie

AU - Champagne, Clara

AU - Tong, Liangqin

AU - Zhang, Renli

AU - Zhang, Fuchun

AU - Qin, Cheng Feng

AU - Ma, Ping

AU - Chen, Chun Hong

AU - Liang, Guodong

AU - Liu, Qiyong

AU - Shi, Pei Yong

AU - Cazelles, Bernard

AU - Wang, Penghua

AU - Tian, Huaiyu

AU - Cheng, Gong

N1 - Funding Information: This work was funded by grants from the National Key Research and Development Plan of China (2018ZX09711003-004-003, 2018YFA0507202, 2016YFD0500400, 2017ZX10305501-003), the Natural Science Foundation of China (31825001, 81730063, 81571975, 81673234), the Shenzhen San-Ming Project for prevention and research on vector-borne diseases (SZSM201611064), and the Municipal Healthcare Joint-Innovation Major Project of Guangzhou (201704020229). P.W. is supported by a National Institutes of Health award R01AI132526. We thank that Professor Scott Weaver from the UTMB provided critical suggestions for the manuscript. G.C. is a Newton Advanced Fellow awarded by the Academy of Medical Sciences and the Newton Fund. We also thank the core facilities of the Center for Life Sciences and Center of Biomedical Analysis for technical assistance (Tsinghua University). Publisher Copyright: © 2019, The Author(s).

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Zika virus (ZIKV) is a mosquito-borne flavivirus that predominantly circulates between humans and Aedes mosquitoes. Clinical studies have shown that Zika viruria in patients persists for an extended period, and results in infectious virions being excreted. Here, we demonstrate that Aedes mosquitoes are permissive to ZIKV infection when breeding in urine or sewage containing low concentrations of ZIKV. Mosquito larvae and pupae, including from field Aedes aegypti can acquire ZIKV from contaminated aquatic systems, resulting in ZIKV infection of adult females. Adult mosquitoes can transmit infectious virions to susceptible type I/II interferon receptor-deficient (ifnagr-/-) C57BL/6 (AG6) mice. Furthermore, ZIKV viruria from infected AG6 mice can causes mosquito infection during the aquatic life stages. Our studies suggest that infectious urine could be a natural ZIKV source, which is potentially transmissible to mosquitoes when breeding in an aquatic environment.

AB - Zika virus (ZIKV) is a mosquito-borne flavivirus that predominantly circulates between humans and Aedes mosquitoes. Clinical studies have shown that Zika viruria in patients persists for an extended period, and results in infectious virions being excreted. Here, we demonstrate that Aedes mosquitoes are permissive to ZIKV infection when breeding in urine or sewage containing low concentrations of ZIKV. Mosquito larvae and pupae, including from field Aedes aegypti can acquire ZIKV from contaminated aquatic systems, resulting in ZIKV infection of adult females. Adult mosquitoes can transmit infectious virions to susceptible type I/II interferon receptor-deficient (ifnagr-/-) C57BL/6 (AG6) mice. Furthermore, ZIKV viruria from infected AG6 mice can causes mosquito infection during the aquatic life stages. Our studies suggest that infectious urine could be a natural ZIKV source, which is potentially transmissible to mosquitoes when breeding in an aquatic environment.

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VL - 10

JO - Nature Communications

JF - Nature Communications

IS - 1

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ER -

Aedes mosquitoes acquire and transmit Zika virus by breeding in contaminated aquatic environments

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