@article{3b88c2bf9d8e4f82b3061d935a0d5954,
title = " Aedes mosquitoes acquire and transmit Zika virus by breeding in contaminated aquatic environments",
abstract = "Zika virus (ZIKV) is a mosquito-borne flavivirus that predominantly circulates between humans and Aedes mosquitoes. Clinical studies have shown that Zika viruria in patients persists for an extended period, and results in infectious virions being excreted. Here, we demonstrate that Aedes mosquitoes are permissive to ZIKV infection when breeding in urine or sewage containing low concentrations of ZIKV. Mosquito larvae and pupae, including from field Aedes aegypti can acquire ZIKV from contaminated aquatic systems, resulting in ZIKV infection of adult females. Adult mosquitoes can transmit infectious virions to susceptible type I/II interferon receptor-deficient (ifnagr-/-) C57BL/6 (AG6) mice. Furthermore, ZIKV viruria from infected AG6 mice can causes mosquito infection during the aquatic life stages. Our studies suggest that infectious urine could be a natural ZIKV source, which is potentially transmissible to mosquitoes when breeding in an aquatic environment.",
author = "Senyan Du and Yang Liu and Jianying Liu and Jie Zhao and Clara Champagne and Liangqin Tong and Renli Zhang and Fuchun Zhang and Qin, {Cheng Feng} and Ping Ma and Chen, {Chun Hong} and Guodong Liang and Qiyong Liu and Shi, {Pei Yong} and Bernard Cazelles and Penghua Wang and Huaiyu Tian and Gong Cheng",
note = "Funding Information: This work was funded by grants from the National Key Research and Development Plan of China (2018ZX09711003-004-003, 2018YFA0507202, 2016YFD0500400, 2017ZX10305501-003), the Natural Science Foundation of China (31825001, 81730063, 81571975, 81673234), the Shenzhen San-Ming Project for prevention and research on vector-borne diseases (SZSM201611064), and the Municipal Healthcare Joint-Innovation Major Project of Guangzhou (201704020229). P.W. is supported by a National Institutes of Health award R01AI132526. We thank that Professor Scott Weaver from the UTMB provided critical suggestions for the manuscript. G.C. is a Newton Advanced Fellow awarded by the Academy of Medical Sciences and the Newton Fund. We also thank the core facilities of the Center for Life Sciences and Center of Biomedical Analysis for technical assistance (Tsinghua University). Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41467-019-09256-0",
language = "English (US)",
volume = "10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}