Age-associated changes in gene expression patterns in the duodenum and colon of rats

Heung Man Lee, George H. Greeley, Ella Englander

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In humans, decreased intestinal motility, compromised nutritional status and increased risk of colon cancer are commonly associated with aging. Here, we used the cDNA microarray analysis to detect age-associated changes in duodenal and colonic gene expression in male Fischer 344 rats. The primary finding of this study is that the magnitude and direction of age-associated changes in gene expression differs in the colon and duodenum. In the colon, 56 genes showed altered expression, whereas expression of only 25 genes was altered in the duodenum. The magnitude of change was greater in the colon than in the duodenum. The direction of change also differed; in the aged colon, expression of 51 genes increased and only five genes decreased. In contrast, in the aged duodenum, only seven genes increased, whereas 18 genes decreased in expression. In the duodenum of aged rats, expression of genes involved in ATP-generating pathways is decreased. In contrast, in the colon of aged rats, expression of genes involved in energy generating pathways and in lipid oxidation is increased. In addition, in the aging colon, an increased expression of genes that show an aberrant regulation in colon cancer, including CD44, ras, and maspin is observed. Collectively, these findings provide clues to molecular events that may be related to compromised intestinal function and the high incidence of colon cancer in the aged population.

Original languageEnglish (US)
Pages (from-to)355-371
Number of pages17
JournalMechanisms of Ageing and Development
Volume122
Issue number4
DOIs
StatePublished - Apr 15 2001

Fingerprint

Duodenum
Gene expression
Rats
Colon
Genes
Gene Expression
Colonic Neoplasms
Gastrointestinal Motility
Inbred F344 Rats
Microarray Analysis
Aging of materials
Oligonucleotide Array Sequence Analysis
Nutritional Status
Adenosine Triphosphate
Microarrays
Lipids
Incidence
Complementary DNA
Population
Oxidation

Keywords

  • Aging
  • cDNA expression array
  • Intestine
  • Rat

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Age-associated changes in gene expression patterns in the duodenum and colon of rats. / Lee, Heung Man; Greeley, George H.; Englander, Ella.

In: Mechanisms of Ageing and Development, Vol. 122, No. 4, 15.04.2001, p. 355-371.

Research output: Contribution to journalArticle

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