Abstract
8-Oxo-7,8-dihydroguanine (8-oxoG) accumulates in the genome over time and is believed to contribute to the development of aging characteristics of skeletal muscle and various aging-related diseases. Here, we show a significantly increased level of intrahelical 8-oxoG and 8-oxoguanine-DNA glycosylase (OGG1) expression in aged human skeletal muscle compared to that of young individuals. In response to exercise, the 8-oxoG level was lastingly elevated in sedentary young and old subjects, but returned rapidly to preexercise levels in the DNA of physically active individuals independent of age. 8-OxoG levels in DNA were inversely correlated with the abundance of acetylated OGG1 (Ac-OGG1), but not with total OGG1, apurinic/apyrimidinic endonuclease 1 (APE1), or Ac-APE1. The actual Ac-OGG1 level was linked to exercise-induced oxidative stress, as shown by changes in lipid peroxide levels and expression of Cu,Zn-SOD, Mn-SOD, and SIRT3, as well as the balance between acetyltransferase p300/CBP and deacetylase SIRT1, but not SIRT6 expression. Together these data suggest that that acetylated form of OGG1, and not OGG1 itself, correlates inversely with the 8-oxoG level in the DNA of human skeletal muscle, and the Ac-OGG1 level is dependent on adaptive cellular responses to physical activity, but is age independent.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 417-423 |
| Number of pages | 7 |
| Journal | Free Radical Biology and Medicine |
| Volume | 51 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 15 2011 |
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Keywords
- 8-OxoG
- Acetylation
- Aging
- Antioxidants
- DNA damage/repair
- Exercise
- Free radicals
- OGG1
- Sirtuins
ASJC Scopus subject areas
- Biochemistry
- Physiology (medical)
Cite this
Age-dependent changes in 8-oxoguanine-DNA glycosylase activity are modulated by adaptive responses to physical exercise in human skeletal muscle. / Radak, Zsolt; Bori, Zoltan; Koltai, Erika; Fatouros, Ioannis G.; Jamurtas, Athanasios Z.; Douroudos, Ioannis I.; Terzis, Gerasimos; Nikolaidis, Michalis G.; Chatzinikolaou, Athanasios; Sovatzidis, Apostolos; Kumagai, Shuzo; Naito, Hisahi; Boldogh, Istvan.
In: Free Radical Biology and Medicine, Vol. 51, No. 2, 15.07.2011, p. 417-423.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Age-dependent changes in 8-oxoguanine-DNA glycosylase activity are modulated by adaptive responses to physical exercise in human skeletal muscle
AU - Radak, Zsolt
AU - Bori, Zoltan
AU - Koltai, Erika
AU - Fatouros, Ioannis G.
AU - Jamurtas, Athanasios Z.
AU - Douroudos, Ioannis I.
AU - Terzis, Gerasimos
AU - Nikolaidis, Michalis G.
AU - Chatzinikolaou, Athanasios
AU - Sovatzidis, Apostolos
AU - Kumagai, Shuzo
AU - Naito, Hisahi
AU - Boldogh, Istvan
PY - 2011/7/15
Y1 - 2011/7/15
N2 - 8-Oxo-7,8-dihydroguanine (8-oxoG) accumulates in the genome over time and is believed to contribute to the development of aging characteristics of skeletal muscle and various aging-related diseases. Here, we show a significantly increased level of intrahelical 8-oxoG and 8-oxoguanine-DNA glycosylase (OGG1) expression in aged human skeletal muscle compared to that of young individuals. In response to exercise, the 8-oxoG level was lastingly elevated in sedentary young and old subjects, but returned rapidly to preexercise levels in the DNA of physically active individuals independent of age. 8-OxoG levels in DNA were inversely correlated with the abundance of acetylated OGG1 (Ac-OGG1), but not with total OGG1, apurinic/apyrimidinic endonuclease 1 (APE1), or Ac-APE1. The actual Ac-OGG1 level was linked to exercise-induced oxidative stress, as shown by changes in lipid peroxide levels and expression of Cu,Zn-SOD, Mn-SOD, and SIRT3, as well as the balance between acetyltransferase p300/CBP and deacetylase SIRT1, but not SIRT6 expression. Together these data suggest that that acetylated form of OGG1, and not OGG1 itself, correlates inversely with the 8-oxoG level in the DNA of human skeletal muscle, and the Ac-OGG1 level is dependent on adaptive cellular responses to physical activity, but is age independent.
AB - 8-Oxo-7,8-dihydroguanine (8-oxoG) accumulates in the genome over time and is believed to contribute to the development of aging characteristics of skeletal muscle and various aging-related diseases. Here, we show a significantly increased level of intrahelical 8-oxoG and 8-oxoguanine-DNA glycosylase (OGG1) expression in aged human skeletal muscle compared to that of young individuals. In response to exercise, the 8-oxoG level was lastingly elevated in sedentary young and old subjects, but returned rapidly to preexercise levels in the DNA of physically active individuals independent of age. 8-OxoG levels in DNA were inversely correlated with the abundance of acetylated OGG1 (Ac-OGG1), but not with total OGG1, apurinic/apyrimidinic endonuclease 1 (APE1), or Ac-APE1. The actual Ac-OGG1 level was linked to exercise-induced oxidative stress, as shown by changes in lipid peroxide levels and expression of Cu,Zn-SOD, Mn-SOD, and SIRT3, as well as the balance between acetyltransferase p300/CBP and deacetylase SIRT1, but not SIRT6 expression. Together these data suggest that that acetylated form of OGG1, and not OGG1 itself, correlates inversely with the 8-oxoG level in the DNA of human skeletal muscle, and the Ac-OGG1 level is dependent on adaptive cellular responses to physical activity, but is age independent.
KW - 8-OxoG
KW - Acetylation
KW - Aging
KW - Antioxidants
KW - DNA damage/repair
KW - Exercise
KW - Free radicals
KW - OGG1
KW - Sirtuins
UR - http://www.scopus.com/inward/record.url?scp=79959347376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79959347376&partnerID=8YFLogxK
U2 - 10.1016/j.freeradbiomed.2011.04.018
DO - 10.1016/j.freeradbiomed.2011.04.018
M3 - Article
C2 - 21569841
AN - SCOPUS:79959347376
VL - 51
SP - 417
EP - 423
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
IS - 2
ER -