Age-dependent modulation of DNA repair enzymes by covalent modification and subcellular distribution

Bartosz Szczesny, Kishor K. Bhakat, Sankar Mitra, Istvan Boldogh

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Chronic oxidative stress is generally believed to be a major etiologic factor in the aging process. In addition to modulation of signaling processes and oxidation of cellular proteins and lipids, reactive oxygen species (ROS) induce multiple damages in both nuclear and mitochondrial genomes, most of which are repaired via the DNA base excision repair pathway. 8-Oxoguanine (8-oxoG), a major ROS product in the genome, is excised by 8-oxoG-DNA glycosylase (OGG1) and the resulting abasic (AP) site is cleaved by AP-endonuclease (APE1) in the initial steps of repair. Here, we provide data showing that differences between young and aged cells' efficiency in import of OGG1 and APE1 may be responsible for age-associated increase in DNA damage in both nuclear and mitochondrial compartments. It is also evident that age-dependent changes in covalent modifications of APE1 by acetylation regulate its action as a transcriptional repressor of many Ca 2+-responsive genes by binding to nCaRE, in addition to its endonuclease activity. Thus, ROS-induced altered signaling is responsible for age-dependent changes in post-translational modifications and import of DNA repair enzymes into nuclei and mitochondria (mt), which in turn affect repair of their genomes.

Original languageEnglish (US)
Pages (from-to)755-765
Number of pages11
JournalMechanisms of Ageing and Development
Volume125
Issue number10-11 SPEC. ISS.
DOIs
StatePublished - Oct 2004

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DNA Repair Enzymes
Reactive Oxygen Species
Genes
Modulation
Repair
DNA-(Apurinic or Apyrimidinic Site) Lyase
Genome
DNA Glycosylases
Mitochondrial Genome
Endonucleases
Post Translational Protein Processing
Acetylation
DNA Repair
DNA Damage
Mitochondria
Oxidative stress
DNA
Oxidative Stress
Lipids
Aging of materials

Keywords

  • 8-Oxoguanine-DNA glycosylase
  • Aging
  • AP-endonuclease
  • DNA base excision repair
  • Organelle targeting
  • Oxidative DNA damage

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Age-dependent modulation of DNA repair enzymes by covalent modification and subcellular distribution. / Szczesny, Bartosz; Bhakat, Kishor K.; Mitra, Sankar; Boldogh, Istvan.

In: Mechanisms of Ageing and Development, Vol. 125, No. 10-11 SPEC. ISS., 10.2004, p. 755-765.

Research output: Contribution to journalArticle

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