Age-related alterations in oxidatively damaged proteins of mouse skeletal muscle mitochondrial electron transport chain complexes

Kashyap B. Choksi, Jonathan E. Nuss, James H. DeFord, John Papaconstantinou

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Age-associated mitochondrial dysfunction is a major source of reactive oxygen species (ROS) and oxidative modification to proteins. Mitochondrial electron transport chain (ETC) complexes I and III are the sites of ROS production and we hypothesize that proteins of the ETC complexes are primary targets of ROS-mediated modification which impairs their structure and function. The pectoralis, primarily an aerobic red muscle, and quadriceps, primarily an anaerobic white muscle, have different rates of respiration and oxygen-carrying capacity, and hence, different rates of ROS production. This raises the question of whether these muscles exhibit different levels of oxidative protein modification. Our studies reveal that the pectoralis shows a dramatic age-related decline in almost all complex activities that correlates with increased oxidative modification. Similar complex proteins were modified in the quadriceps, at a significantly lower level with less change in enzyme and ETC coupling function. We postulate that mitochondrial ROS causes damage to specific ETC subunits which increases with age and leads to further mitochondrial dysfunction. We conclude that physiological characteristics of the pectoralis vs quadriceps may play a role in age-associated rate of mitochondrial dysfunction and in the decline in tissue function.

Original languageEnglish (US)
Pages (from-to)826-838
Number of pages13
JournalFree Radical Biology and Medicine
Volume45
Issue number6
DOIs
StatePublished - Sep 15 2008

Fingerprint

Electron Transport
Muscle
Reactive Oxygen Species
Skeletal Muscle
Proteins
Electron Transport Chain Complex Proteins
Electron Transport Complex I
Muscles
Electron Transport Complex III
Quadriceps Muscle
Conservation of Natural Resources
Respiratory Rate
Tissue
Oxygen
Enzymes

Keywords

  • 4-Hydroxynonenal
  • Aging
  • Carbonylation
  • Mitochondrial dysfunction
  • Oxidative stress
  • Pectoralis
  • Quadriceps
  • Skeletal muscle
  • Tyrosine nitration

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Age-related alterations in oxidatively damaged proteins of mouse skeletal muscle mitochondrial electron transport chain complexes. / Choksi, Kashyap B.; Nuss, Jonathan E.; DeFord, James H.; Papaconstantinou, John.

In: Free Radical Biology and Medicine, Vol. 45, No. 6, 15.09.2008, p. 826-838.

Research output: Contribution to journalArticle

@article{17666dd5041140258db2fb6d9cb07117,
title = "Age-related alterations in oxidatively damaged proteins of mouse skeletal muscle mitochondrial electron transport chain complexes",
abstract = "Age-associated mitochondrial dysfunction is a major source of reactive oxygen species (ROS) and oxidative modification to proteins. Mitochondrial electron transport chain (ETC) complexes I and III are the sites of ROS production and we hypothesize that proteins of the ETC complexes are primary targets of ROS-mediated modification which impairs their structure and function. The pectoralis, primarily an aerobic red muscle, and quadriceps, primarily an anaerobic white muscle, have different rates of respiration and oxygen-carrying capacity, and hence, different rates of ROS production. This raises the question of whether these muscles exhibit different levels of oxidative protein modification. Our studies reveal that the pectoralis shows a dramatic age-related decline in almost all complex activities that correlates with increased oxidative modification. Similar complex proteins were modified in the quadriceps, at a significantly lower level with less change in enzyme and ETC coupling function. We postulate that mitochondrial ROS causes damage to specific ETC subunits which increases with age and leads to further mitochondrial dysfunction. We conclude that physiological characteristics of the pectoralis vs quadriceps may play a role in age-associated rate of mitochondrial dysfunction and in the decline in tissue function.",
keywords = "4-Hydroxynonenal, Aging, Carbonylation, Mitochondrial dysfunction, Oxidative stress, Pectoralis, Quadriceps, Skeletal muscle, Tyrosine nitration",
author = "Choksi, {Kashyap B.} and Nuss, {Jonathan E.} and DeFord, {James H.} and John Papaconstantinou",
year = "2008",
month = "9",
day = "15",
doi = "10.1016/j.freeradbiomed.2008.06.006",
language = "English (US)",
volume = "45",
pages = "826--838",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Age-related alterations in oxidatively damaged proteins of mouse skeletal muscle mitochondrial electron transport chain complexes

AU - Choksi, Kashyap B.

AU - Nuss, Jonathan E.

AU - DeFord, James H.

AU - Papaconstantinou, John

PY - 2008/9/15

Y1 - 2008/9/15

N2 - Age-associated mitochondrial dysfunction is a major source of reactive oxygen species (ROS) and oxidative modification to proteins. Mitochondrial electron transport chain (ETC) complexes I and III are the sites of ROS production and we hypothesize that proteins of the ETC complexes are primary targets of ROS-mediated modification which impairs their structure and function. The pectoralis, primarily an aerobic red muscle, and quadriceps, primarily an anaerobic white muscle, have different rates of respiration and oxygen-carrying capacity, and hence, different rates of ROS production. This raises the question of whether these muscles exhibit different levels of oxidative protein modification. Our studies reveal that the pectoralis shows a dramatic age-related decline in almost all complex activities that correlates with increased oxidative modification. Similar complex proteins were modified in the quadriceps, at a significantly lower level with less change in enzyme and ETC coupling function. We postulate that mitochondrial ROS causes damage to specific ETC subunits which increases with age and leads to further mitochondrial dysfunction. We conclude that physiological characteristics of the pectoralis vs quadriceps may play a role in age-associated rate of mitochondrial dysfunction and in the decline in tissue function.

AB - Age-associated mitochondrial dysfunction is a major source of reactive oxygen species (ROS) and oxidative modification to proteins. Mitochondrial electron transport chain (ETC) complexes I and III are the sites of ROS production and we hypothesize that proteins of the ETC complexes are primary targets of ROS-mediated modification which impairs their structure and function. The pectoralis, primarily an aerobic red muscle, and quadriceps, primarily an anaerobic white muscle, have different rates of respiration and oxygen-carrying capacity, and hence, different rates of ROS production. This raises the question of whether these muscles exhibit different levels of oxidative protein modification. Our studies reveal that the pectoralis shows a dramatic age-related decline in almost all complex activities that correlates with increased oxidative modification. Similar complex proteins were modified in the quadriceps, at a significantly lower level with less change in enzyme and ETC coupling function. We postulate that mitochondrial ROS causes damage to specific ETC subunits which increases with age and leads to further mitochondrial dysfunction. We conclude that physiological characteristics of the pectoralis vs quadriceps may play a role in age-associated rate of mitochondrial dysfunction and in the decline in tissue function.

KW - 4-Hydroxynonenal

KW - Aging

KW - Carbonylation

KW - Mitochondrial dysfunction

KW - Oxidative stress

KW - Pectoralis

KW - Quadriceps

KW - Skeletal muscle

KW - Tyrosine nitration

UR - http://www.scopus.com/inward/record.url?scp=55249104165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=55249104165&partnerID=8YFLogxK

U2 - 10.1016/j.freeradbiomed.2008.06.006

DO - 10.1016/j.freeradbiomed.2008.06.006

M3 - Article

C2 - 18598756

AN - SCOPUS:55249104165

VL - 45

SP - 826

EP - 838

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - 6

ER -