Age-related changes in polyamine biosynthesis after fasting and refeeding

Kiego Yoshinaga, Jin Ishizuka, B. Mark Evers, Courtney Townsend, James C. Thompson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The effect of aging on ornithine decarboxylase (ODC) activity and polyamine biosynthesis in the proximal small intestine was studied in two groups of male Fisher 344 rats (young [4-month old] and aged [26- to 27-month old]) using a fasting and refeeding model. In control (nonfasted) rats, levels of polyamines (putrescine, spermidine and spermine) and ODC activity were significantly higher in aged compared with young rats. In aged rats, fasting significantly reduced the levels of putrescine by 41%, spermidine by 23%, and spermine by 11%; however, fasting had no effect on polyamine levels in young rats. ODC activity was decreased 75% in young and 50% in aged rats after fasting compared with the respective age-matched controls. Conversely, 2 h after reinstituting a chow diet increased ODC activity by 17-fold in young rats but only 8-fold in aged rats. Putrescine levels were also increased in both age groups after refeeding; however, similar to ODC activity, these increases were much less in aged rats. In addition, spermidine and spermine levels remained significantly depressed in the aged groups even after 24 h of refeeding. These findings suggest that the normal rigid control of gut polyamine biosynthesis and proliferation noted in young rats is markedly altered with aging.

Original languageEnglish (US)
Pages (from-to)565-572
Number of pages8
JournalExperimental Gerontology
Volume28
Issue number6
DOIs
StatePublished - 1993

Fingerprint

Biosynthesis
Polyamines
Rats
Fasting
Ornithine Decarboxylase
Putrescine
Spermidine
Spermine
Aging of materials
Rat control
Nutrition
Small Intestine
Age Groups
Diet

Keywords

  • aging
  • gut mucosa
  • ornithine decarboxylase
  • polyamines
  • proliferation

ASJC Scopus subject areas

  • Aging
  • Medicine(all)

Cite this

Age-related changes in polyamine biosynthesis after fasting and refeeding. / Yoshinaga, Kiego; Ishizuka, Jin; Evers, B. Mark; Townsend, Courtney; Thompson, James C.

In: Experimental Gerontology, Vol. 28, No. 6, 1993, p. 565-572.

Research output: Contribution to journalArticle

Yoshinaga, Kiego ; Ishizuka, Jin ; Evers, B. Mark ; Townsend, Courtney ; Thompson, James C. / Age-related changes in polyamine biosynthesis after fasting and refeeding. In: Experimental Gerontology. 1993 ; Vol. 28, No. 6. pp. 565-572.
@article{822ce1180ea14fbbb62fa49523351955,
title = "Age-related changes in polyamine biosynthesis after fasting and refeeding",
abstract = "The effect of aging on ornithine decarboxylase (ODC) activity and polyamine biosynthesis in the proximal small intestine was studied in two groups of male Fisher 344 rats (young [4-month old] and aged [26- to 27-month old]) using a fasting and refeeding model. In control (nonfasted) rats, levels of polyamines (putrescine, spermidine and spermine) and ODC activity were significantly higher in aged compared with young rats. In aged rats, fasting significantly reduced the levels of putrescine by 41{\%}, spermidine by 23{\%}, and spermine by 11{\%}; however, fasting had no effect on polyamine levels in young rats. ODC activity was decreased 75{\%} in young and 50{\%} in aged rats after fasting compared with the respective age-matched controls. Conversely, 2 h after reinstituting a chow diet increased ODC activity by 17-fold in young rats but only 8-fold in aged rats. Putrescine levels were also increased in both age groups after refeeding; however, similar to ODC activity, these increases were much less in aged rats. In addition, spermidine and spermine levels remained significantly depressed in the aged groups even after 24 h of refeeding. These findings suggest that the normal rigid control of gut polyamine biosynthesis and proliferation noted in young rats is markedly altered with aging.",
keywords = "aging, gut mucosa, ornithine decarboxylase, polyamines, proliferation",
author = "Kiego Yoshinaga and Jin Ishizuka and Evers, {B. Mark} and Courtney Townsend and Thompson, {James C.}",
year = "1993",
doi = "10.1016/0531-5565(93)90045-F",
language = "English (US)",
volume = "28",
pages = "565--572",
journal = "Experimental Gerontology",
issn = "0531-5565",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Age-related changes in polyamine biosynthesis after fasting and refeeding

AU - Yoshinaga, Kiego

AU - Ishizuka, Jin

AU - Evers, B. Mark

AU - Townsend, Courtney

AU - Thompson, James C.

PY - 1993

Y1 - 1993

N2 - The effect of aging on ornithine decarboxylase (ODC) activity and polyamine biosynthesis in the proximal small intestine was studied in two groups of male Fisher 344 rats (young [4-month old] and aged [26- to 27-month old]) using a fasting and refeeding model. In control (nonfasted) rats, levels of polyamines (putrescine, spermidine and spermine) and ODC activity were significantly higher in aged compared with young rats. In aged rats, fasting significantly reduced the levels of putrescine by 41%, spermidine by 23%, and spermine by 11%; however, fasting had no effect on polyamine levels in young rats. ODC activity was decreased 75% in young and 50% in aged rats after fasting compared with the respective age-matched controls. Conversely, 2 h after reinstituting a chow diet increased ODC activity by 17-fold in young rats but only 8-fold in aged rats. Putrescine levels were also increased in both age groups after refeeding; however, similar to ODC activity, these increases were much less in aged rats. In addition, spermidine and spermine levels remained significantly depressed in the aged groups even after 24 h of refeeding. These findings suggest that the normal rigid control of gut polyamine biosynthesis and proliferation noted in young rats is markedly altered with aging.

AB - The effect of aging on ornithine decarboxylase (ODC) activity and polyamine biosynthesis in the proximal small intestine was studied in two groups of male Fisher 344 rats (young [4-month old] and aged [26- to 27-month old]) using a fasting and refeeding model. In control (nonfasted) rats, levels of polyamines (putrescine, spermidine and spermine) and ODC activity were significantly higher in aged compared with young rats. In aged rats, fasting significantly reduced the levels of putrescine by 41%, spermidine by 23%, and spermine by 11%; however, fasting had no effect on polyamine levels in young rats. ODC activity was decreased 75% in young and 50% in aged rats after fasting compared with the respective age-matched controls. Conversely, 2 h after reinstituting a chow diet increased ODC activity by 17-fold in young rats but only 8-fold in aged rats. Putrescine levels were also increased in both age groups after refeeding; however, similar to ODC activity, these increases were much less in aged rats. In addition, spermidine and spermine levels remained significantly depressed in the aged groups even after 24 h of refeeding. These findings suggest that the normal rigid control of gut polyamine biosynthesis and proliferation noted in young rats is markedly altered with aging.

KW - aging

KW - gut mucosa

KW - ornithine decarboxylase

KW - polyamines

KW - proliferation

UR - http://www.scopus.com/inward/record.url?scp=0027753789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027753789&partnerID=8YFLogxK

U2 - 10.1016/0531-5565(93)90045-F

DO - 10.1016/0531-5565(93)90045-F

M3 - Article

VL - 28

SP - 565

EP - 572

JO - Experimental Gerontology

JF - Experimental Gerontology

SN - 0531-5565

IS - 6

ER -