Agglutination of bacteria and erythrocytes by serum from six species of marine molluscs

William S. Fisher, Anthony R. DiNuzzo

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Lectins have been implicated as recognition molecules in the invertebrate immune response, yet their capacity to recognize (agglutinate and/or opsonize) potentially invasive microorganisms is largely unknown. In this study, sera from six species of marine molluscs (oyster, clam, octopus, squid, cuttlefish, and sea hare) were found to agglutinate 64 of 94 bacterial isolates (15 genera, 36 species) and seven types of vertebrate erythrocytes. Oyster, clam, and octopus sera agglutinated the greatest number of bacterial isolates and oyster serum exhibited the highest intensity and titer of agglutination. Agglutination was isolate dependent, implying high lectin specificity. Titers were highly variable for wild populations of oysters and sea hares and relatively constant for F4-generation cuttlefish reared in aquaculture systems. Simultaneous agglutination of specific bacterial isolates indicated conservation of lectin specificity between oyster and clam sera (bivalves) and between squid and cuttlefish sera (cephalopods).

Original languageEnglish (US)
Pages (from-to)380-394
Number of pages15
JournalJournal of Invertebrate Pathology
Volume57
Issue number3
DOIs
StatePublished - 1991

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Keywords

  • Agglutination
  • hemagglutination
  • invertebrate immunology
  • lectins
  • marine molluscs
  • nonself recognition

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

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