Aldose and aldehyde reductases in human tissues

Satish Srivastava, Naseem Ansari, Gregory A. Hair, Ballabh Das

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Immunochemical characterizations of aldose reductase and aldehyde reductases I and II, partially purified by DEAE-cellulose (DE-52) column chromatography from human tissues, were carried out by immunotitration, using antisera raised against the homogenous preparations of human and bovine lens aldose reductase and human placenta aldehyde reductase I and aldehyde reductase II. Anti-aldose reductase antiserum cross-reacted with aldehyde reductase I, anti-aldehyde reductase I antiserum cross-reacted with aldose reductase and anti-aldehyde reductase II antiserum precipitated aldehyde reductase II, but did not cross-react with aldose reductase or aldehyde reductase I from all the tissues examined. DE-52 elution profiles, substrate specificity and immunochemical characterization indicate that aldose reductase is present in human aorta, brain, erythrocyte and muscle; aldehyde reductase I is present in human kidney, liver and placenta; and aldehyde reductase II is present in human brain, erythrocyte, kidney, liver, lung and placenta. Monospecific anti-α and anti-β antisera were purified from placenta anti-aldehyde reductase I antiserum, using immunoaffinity techniques. Anti-α antiserum precipitated both aldehyde reductase I and aldose reductase, whereas anti-β antibodies cross-reacted with only aldehyde reductase I. Based on these studies, a three gene loci model is proposed to explain the genetic interrelationships among these enzymes. Aldose reductase is a monomer of α subunits, aldehyde reductase I is a dimer of α and β subunits and aldehyde reductase II is a monomer of δ subunits.

Original languageEnglish (US)
Pages (from-to)220-227
Number of pages8
JournalBBA - General Subjects
Volume800
Issue number3
DOIs
StatePublished - Aug 21 1984

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Aldehyde Reductase
L-glucuronate reductase
Tissue
Immune Sera
Placenta
Liver
Brain
Erythrocytes
Monomers
Kidney
DEAE-Cellulose
Column chromatography

Keywords

  • (Human)
  • Aldehyde reductase
  • Aldose reductase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Aldose and aldehyde reductases in human tissues. / Srivastava, Satish; Ansari, Naseem; Hair, Gregory A.; Das, Ballabh.

In: BBA - General Subjects, Vol. 800, No. 3, 21.08.1984, p. 220-227.

Research output: Contribution to journalArticle

Srivastava, Satish ; Ansari, Naseem ; Hair, Gregory A. ; Das, Ballabh. / Aldose and aldehyde reductases in human tissues. In: BBA - General Subjects. 1984 ; Vol. 800, No. 3. pp. 220-227.
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