Aldose reductase inhibition prevents endotoxin-induced uveitis in rats

Umesh C S Yadav, Satish Srivastava, Kota Ramana

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

PURPOSE. The purpose of the present study was to elucidate the role of the polyol pathway enzyme aldose reductase (AR) in the mediation of ocular inflammation in a rat model of endotoxin-induced uveitis (EIU). METHODS. EIU was induced by a subcutaneous injection of 200 μg lipopolysaccharide (LPS) in male Lewis rats treated with the AR inhibitor, zopolrestat (25 mg/kg body weight, intraperitoneally) or its carrier. The rats were killed 24 hours after LPS injection, the eyes were enucleated immediately, and aqueous humor (AqH) was collected. The number of infiltrating cells, protein concentration, and levels of nitric oxide (NO), tumor necrosis factor (TNF)-α, and prostaglandin E2 (PGE2) in the AqH were determined. Immunohistochemical analysis was performed in paraformaldehyde-fixed eye sections by staining with antibodies against iNOS, COX-2, TNF-α, NF-κB, and AR. The levels of reactive oxygen species (ROS) in rat eye sections were determined by dihydroethidium (hydroethidine) fluorescence staining. RESULTS. In the EIU rat eye AqH, both the number of infiltrating cells and protein concentrations of the inflammatory markers, TNF-α, NO, and PGE2 were significantly higher than in the control rats, and inhibition of AR by zopolrestat suppressed the LPS-induced increases. The LPS-induced increased expression of AR, TNF-α, iNOS, and COX-2 proteins in the ciliary body, corneal epithelium, and retinal wall was also significantly inhibited by zopolrestat. Furthermore, AR inhibition prevented the LPS-induced increased levels of ROS and activation of NF-κB in the ciliary body, corneal epithelium, and retinal wall of the rat eye. AR inhibition also prevented the LPS-induced activation of NF-κB and expression of COX-2 and iNOS in the human monocyte cell line U-937. CONCLUSIONS. The results indicate that AR inhibition suppresses the inflammation in EIU by blocking the expression and release of inflammatory markers in ocular tissues, along with the attenuation of NF-κB activation. This finding suggests that AR inhibition could be a novel therapeutic target for the treatment of uveitis and associated ocular inflammation.

Original languageEnglish (US)
Pages (from-to)4634-4642
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume48
Issue number10
DOIs
StatePublished - Oct 2007

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Aldehyde Reductase
Uveitis
Endotoxins
Lipopolysaccharides
Aqueous Humor
Tumor Necrosis Factor-alpha
Corneal Epithelium
Ciliary Body
Inflammation
Dinoprostone
Reactive Oxygen Species
Nitric Oxide
Cell Count
Staining and Labeling
Proteins
Subcutaneous Injections
Monocytes
Fluorescence
Body Weight
Cell Line

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Aldose reductase inhibition prevents endotoxin-induced uveitis in rats. / Yadav, Umesh C S; Srivastava, Satish; Ramana, Kota.

In: Investigative Ophthalmology and Visual Science, Vol. 48, No. 10, 10.2007, p. 4634-4642.

Research output: Contribution to journalArticle

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abstract = "PURPOSE. The purpose of the present study was to elucidate the role of the polyol pathway enzyme aldose reductase (AR) in the mediation of ocular inflammation in a rat model of endotoxin-induced uveitis (EIU). METHODS. EIU was induced by a subcutaneous injection of 200 μg lipopolysaccharide (LPS) in male Lewis rats treated with the AR inhibitor, zopolrestat (25 mg/kg body weight, intraperitoneally) or its carrier. The rats were killed 24 hours after LPS injection, the eyes were enucleated immediately, and aqueous humor (AqH) was collected. The number of infiltrating cells, protein concentration, and levels of nitric oxide (NO), tumor necrosis factor (TNF)-α, and prostaglandin E2 (PGE2) in the AqH were determined. Immunohistochemical analysis was performed in paraformaldehyde-fixed eye sections by staining with antibodies against iNOS, COX-2, TNF-α, NF-κB, and AR. The levels of reactive oxygen species (ROS) in rat eye sections were determined by dihydroethidium (hydroethidine) fluorescence staining. RESULTS. In the EIU rat eye AqH, both the number of infiltrating cells and protein concentrations of the inflammatory markers, TNF-α, NO, and PGE2 were significantly higher than in the control rats, and inhibition of AR by zopolrestat suppressed the LPS-induced increases. The LPS-induced increased expression of AR, TNF-α, iNOS, and COX-2 proteins in the ciliary body, corneal epithelium, and retinal wall was also significantly inhibited by zopolrestat. Furthermore, AR inhibition prevented the LPS-induced increased levels of ROS and activation of NF-κB in the ciliary body, corneal epithelium, and retinal wall of the rat eye. AR inhibition also prevented the LPS-induced activation of NF-κB and expression of COX-2 and iNOS in the human monocyte cell line U-937. CONCLUSIONS. The results indicate that AR inhibition suppresses the inflammation in EIU by blocking the expression and release of inflammatory markers in ocular tissues, along with the attenuation of NF-κB activation. This finding suggests that AR inhibition could be a novel therapeutic target for the treatment of uveitis and associated ocular inflammation.",
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