Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells

Ravinder Tammali, Kota Ramana, Sharad S. Singhal, Sanjay Awasthi, Satish Srivastava

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Inhibition of prostaglandin E2 (PGE2) and cyclooxygenase (COX)-2 by nonsteroidal anti-inflammatory drugs reduces the progression of colon cancer. Inhibition of aldose reductase (AR; EC. 1.1.1.21.) by sorbinil or by antisense ablation prevented fibroblast growth factor-induced and platelet-derived growth factor-induced up-regulation of PGE2 synthesis in human colon cancer cells, Caco-2. AR besides reducing aldo-sugars efficiently reduces toxic lipid aldehydes and their conjugates with glutathione. Inhibition of AR prevented growth factor-induced COX-2 activity, protein, and mRNA and significantly decreased activation of nuclear factor-κB and protein kinase C (PKC) and phosphorylation of PKC-β2 as well as progression of Caco-2 cell growth but had no effect on COX-1 activity. Cell cycle analysis suggests that inhibition of AR prevents growth factor-induced proliferation of Caco-2 cells at S phase. Treatment of Caco-2 cells with the most abundant and toxic lipid aldehyde 4-hydroxy-trans-2-nonenal (HNE) or its glutathione- conjugate [glutathionyl-HNE (GS-HNE)] or AR-catalyzed product of GS-HNE, glutathionyl-1,4-dihydroxynonane (GS-DHN), resulted in increased COX-2 expression and PGE2 production. Inhibition of AR prevented HNE- or GS-HNE-induced but not GS-DHN-induced upregulation of COX-2 and PGE2. More importantly, in vivo studies showed that administration of AR-small interfering RNA (siRNA), but not control siRNA, to nude mice bearing SW480 human colon adenocarcinoma cells completely arrested tumor progression. Collectively, these observations suggest that AR is an obligatory mediator of growth factor-induced up-regulation of COX-2, PGE2, and growth of Caco-2 cells, indicating that inhibition of AR may be a novel therapeutic approach in preventing the progression of colon cancer.

Original languageEnglish (US)
Pages (from-to)9705-9713
Number of pages9
JournalCancer Research
Volume66
Issue number19
DOIs
StatePublished - Oct 1 2006

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Aldehyde Reductase
Cyclooxygenase 2
Caco-2 Cells
Dinoprostone
Colonic Neoplasms
Intercellular Signaling Peptides and Proteins
Up-Regulation
Poisons
Aldehydes
Protein Kinase C
Small Interfering RNA
Glutathione
Lipids
Cyclooxygenase 1
Fibroblast Growth Factors
Platelet-Derived Growth Factor
Prostaglandin-Endoperoxide Synthases
Growth
Nuclear Proteins
S Phase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells. / Tammali, Ravinder; Ramana, Kota; Singhal, Sharad S.; Awasthi, Sanjay; Srivastava, Satish.

In: Cancer Research, Vol. 66, No. 19, 01.10.2006, p. 9705-9713.

Research output: Contribution to journalArticle

Tammali, Ravinder ; Ramana, Kota ; Singhal, Sharad S. ; Awasthi, Sanjay ; Srivastava, Satish. / Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells. In: Cancer Research. 2006 ; Vol. 66, No. 19. pp. 9705-9713.
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