Aldosterone binding sites in aortic cell cultures from spontaneously hypertensive rats

N. R. Nichols, C. E. Hall, Walter Meyer

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Spontaneously hypertensive rats and rats made hypertensive by deoxycorticosterone-salt treatment have in common increased Na+ and K+ permeability and transport in their aortic cells. These changes may be important factors in the development of the hypertensive state and may be mediated by mineralocorticoid binding to intracellular sites in the aorta. Therefore, we examined 3H-aldosterone binding in aortic cell cultures from spontaneously hypertensive rats and normotensive Wistar-Kyoto rats. Vascular corticoid binding sites in the two strains were compared by Scatchard analysis of K(d) and B(max), pH and temperature stability, and subcellular binding. By all of these criteria we found that aldosterone binding sites in cultured aortic cells are similar in the hypertensive and normotensive rats. These results indicate that the underlying genetic defect in spontaneous hypertension is not an intrinsic cellular defect which alters mineralocorticoid binding in the aorta.

Original languageEnglish (US)
Pages (from-to)646-651
Number of pages6
JournalHypertension
Volume4
Issue number5 I
StatePublished - 1982

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Mineralocorticoids
Inbred SHR Rats
Aldosterone
Aorta
Cell Culture Techniques
Binding Sites
Desoxycorticosterone
Inbred WKY Rats
Blood Vessels
Cultured Cells
Permeability
Adrenal Cortex Hormones
Salts
Hypertension
Temperature

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Nichols, N. R., Hall, C. E., & Meyer, W. (1982). Aldosterone binding sites in aortic cell cultures from spontaneously hypertensive rats. Hypertension, 4(5 I), 646-651.

Aldosterone binding sites in aortic cell cultures from spontaneously hypertensive rats. / Nichols, N. R.; Hall, C. E.; Meyer, Walter.

In: Hypertension, Vol. 4, No. 5 I, 1982, p. 646-651.

Research output: Contribution to journalArticle

Nichols, NR, Hall, CE & Meyer, W 1982, 'Aldosterone binding sites in aortic cell cultures from spontaneously hypertensive rats', Hypertension, vol. 4, no. 5 I, pp. 646-651.
Nichols, N. R. ; Hall, C. E. ; Meyer, Walter. / Aldosterone binding sites in aortic cell cultures from spontaneously hypertensive rats. In: Hypertension. 1982 ; Vol. 4, No. 5 I. pp. 646-651.
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