Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response

Renata Polakowska; Giuditta Perozzi; Louise Prakash

doi:10.1007/BF00410912

Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response

Renata Polakowska, Giuditta Perozzi, Louise Prakash

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both stationary and exponential phase cells. These and other observations indicate that the mechanisms of repair of alkylation damage and mutagenesis differ markedly between S. cerevisiae and Escherichia coli.

Original language	English (US)
Pages (from-to)	647-655
Number of pages	9
Journal	Current Genetics
Volume	10
Issue number	9
DOIs	https://doi.org/10.1007/BF00410912
State	Published - May 1986
Externally published	Yes

Keywords

Adaptive response
Alkylation mutagenesis
Yeast
rad52
rad6

ASJC Scopus subject areas

Genetics

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10.1007/BF00410912

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title = "Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response",

abstract = "We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both stationary and exponential phase cells. These and other observations indicate that the mechanisms of repair of alkylation damage and mutagenesis differ markedly between S. cerevisiae and Escherichia coli.",

keywords = "Adaptive response, Alkylation mutagenesis, Yeast, rad52, rad6",

author = "Renata Polakowska and Giuditta Perozzi and Louise Prakash",

year = "1986",

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pages = "647--655",

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AU - Polakowska, Renata

AU - Perozzi, Giuditta

AU - Prakash, Louise

PY - 1986/5

Y1 - 1986/5

N2 - We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both stationary and exponential phase cells. These and other observations indicate that the mechanisms of repair of alkylation damage and mutagenesis differ markedly between S. cerevisiae and Escherichia coli.

AB - We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both stationary and exponential phase cells. These and other observations indicate that the mechanisms of repair of alkylation damage and mutagenesis differ markedly between S. cerevisiae and Escherichia coli.

KW - Adaptive response

KW - Alkylation mutagenesis

KW - Yeast

KW - rad52

KW - rad6

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Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response

Abstract

Keywords

ASJC Scopus subject areas

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