Allergen challenge in asthma

Association of eosinophils and lymphocytes with interleukin-5

Sanjiv Sur, G. J. Gleich, K. P. Offord, M. C. Swanson, T. Ohnishi, L. B. Martin, J. M. Wagner, D. A. Weiler, L. W. Hunt

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To test whether eosinophil recruitment after pulmonary allergen challenge is associated with interleukin (IL)-5 in patients with asthma, we performed segmental bronchoprovocation (SBP) with saline, and with low and high dosages of ragweed extract in six patients with allergic asthma. Bronchoalveolar lavage (BAL) of the challenged segments was performed 5 min after challenge (immediate BAL fluid) and repeated 24 h later (late BAL fluid). Allergen challenge resulted in recruitment of eosinophils, and increased levels of eosinophil-active cytokines. A bioassay showed the predominant eosinophil-active cytokine in the late BAL fluids to be IL-5. Analysis of the late BAL fluids revealed that IL-5 levels correlated with the numbers of eosinophils and lymphocytes. This study provides evidence that IL-5 is a critical cytokine associated with eosinophil and lymphocyte recruitment into the airways of patients with asthma following exposure to allergen.

Original languageEnglish (US)
Pages (from-to)891-898
Number of pages8
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume50
Issue number11
StatePublished - 1995
Externally publishedYes

Fingerprint

Interleukin-5
Eosinophils
Allergens
Asthma
Bronchoalveolar Lavage Fluid
Lymphocytes
Cytokines
Ambrosia
Lymphocyte Count
Bronchoalveolar Lavage
Biological Assay
Lung

Keywords

  • Asthma
  • Bronchoprovocation
  • Eosinophils
  • Interleukin-5
  • Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Sur, S., Gleich, G. J., Offord, K. P., Swanson, M. C., Ohnishi, T., Martin, L. B., ... Hunt, L. W. (1995). Allergen challenge in asthma: Association of eosinophils and lymphocytes with interleukin-5. Allergy: European Journal of Allergy and Clinical Immunology, 50(11), 891-898.

Allergen challenge in asthma : Association of eosinophils and lymphocytes with interleukin-5. / Sur, Sanjiv; Gleich, G. J.; Offord, K. P.; Swanson, M. C.; Ohnishi, T.; Martin, L. B.; Wagner, J. M.; Weiler, D. A.; Hunt, L. W.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 50, No. 11, 1995, p. 891-898.

Research output: Contribution to journalArticle

Sur, S, Gleich, GJ, Offord, KP, Swanson, MC, Ohnishi, T, Martin, LB, Wagner, JM, Weiler, DA & Hunt, LW 1995, 'Allergen challenge in asthma: Association of eosinophils and lymphocytes with interleukin-5', Allergy: European Journal of Allergy and Clinical Immunology, vol. 50, no. 11, pp. 891-898.
Sur, Sanjiv ; Gleich, G. J. ; Offord, K. P. ; Swanson, M. C. ; Ohnishi, T. ; Martin, L. B. ; Wagner, J. M. ; Weiler, D. A. ; Hunt, L. W. / Allergen challenge in asthma : Association of eosinophils and lymphocytes with interleukin-5. In: Allergy: European Journal of Allergy and Clinical Immunology. 1995 ; Vol. 50, No. 11. pp. 891-898.
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AU - Martin, L. B.

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AB - To test whether eosinophil recruitment after pulmonary allergen challenge is associated with interleukin (IL)-5 in patients with asthma, we performed segmental bronchoprovocation (SBP) with saline, and with low and high dosages of ragweed extract in six patients with allergic asthma. Bronchoalveolar lavage (BAL) of the challenged segments was performed 5 min after challenge (immediate BAL fluid) and repeated 24 h later (late BAL fluid). Allergen challenge resulted in recruitment of eosinophils, and increased levels of eosinophil-active cytokines. A bioassay showed the predominant eosinophil-active cytokine in the late BAL fluids to be IL-5. Analysis of the late BAL fluids revealed that IL-5 levels correlated with the numbers of eosinophils and lymphocytes. This study provides evidence that IL-5 is a critical cytokine associated with eosinophil and lymphocyte recruitment into the airways of patients with asthma following exposure to allergen.

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