Allergen-induced recruitment of FcεRI+ eosinophils in human atopic skin

Luis T. Barata, Sun Ying, J. Andrew Grant, Marc Humbert, Julia Barkans, Qui Meng, Stephen R. Durham, A. Barry Kay

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18 Scopus citations

Abstract

We have attempted to identify FcεRI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30% expressed mRNA for the α, β, and γ chains of FcεRI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-a chain antibody, the FcεRIα protein also co-localized to approximately 50-80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of FcεRI+ (997+)/EG2+ eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express FcεRI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo.

Original languageEnglish (US)
Pages (from-to)1236-1241
Number of pages6
JournalEuropean Journal of Immunology
Volume27
Issue number5
StatePublished - Mar 1997

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Keywords

  • Allergy
  • Eosinophils
  • Fc receptors
  • Skin

ASJC Scopus subject areas

  • Immunology

Cite this

Barata, L. T., Ying, S., Grant, J. A., Humbert, M., Barkans, J., Meng, Q., Durham, S. R., & Kay, A. B. (1997). Allergen-induced recruitment of FcεRI+ eosinophils in human atopic skin. European Journal of Immunology, 27(5), 1236-1241.