Allergen-induced recruitment of FcεRI+ eosinophils in human atopic skin

Luis T. Barata, Sun Ying, J. Andrew Grant, Marc Humbert, Julia Barkans, Qui Meng, Stephen R. Durham, A. Barry Kay

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have attempted to identify FcεRI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30% expressed mRNA for the α, β, and γ chains of FcεRI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-a chain antibody, the FcεRIα protein also co-localized to approximately 50-80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of FcεRI+ (997+)/EG2+ eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express FcεRI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo.

Original languageEnglish (US)
Pages (from-to)1236-1241
Number of pages6
JournalEuropean Journal of Immunology
Volume27
Issue number5
StatePublished - Mar 1997

Fingerprint

Eosinophils
Allergens
Skin
IgE Receptors
Secretory Pathway
In Situ Hybridization
Hypersensitivity
Messenger RNA
Injections
Antibodies
Proteins

Keywords

  • Allergy
  • Eosinophils
  • Fc receptors
  • Skin

ASJC Scopus subject areas

  • Immunology

Cite this

Barata, L. T., Ying, S., Grant, J. A., Humbert, M., Barkans, J., Meng, Q., ... Kay, A. B. (1997). Allergen-induced recruitment of FcεRI+ eosinophils in human atopic skin. European Journal of Immunology, 27(5), 1236-1241.

Allergen-induced recruitment of FcεRI+ eosinophils in human atopic skin. / Barata, Luis T.; Ying, Sun; Grant, J. Andrew; Humbert, Marc; Barkans, Julia; Meng, Qui; Durham, Stephen R.; Kay, A. Barry.

In: European Journal of Immunology, Vol. 27, No. 5, 03.1997, p. 1236-1241.

Research output: Contribution to journalArticle

Barata, LT, Ying, S, Grant, JA, Humbert, M, Barkans, J, Meng, Q, Durham, SR & Kay, AB 1997, 'Allergen-induced recruitment of FcεRI+ eosinophils in human atopic skin', European Journal of Immunology, vol. 27, no. 5, pp. 1236-1241.
Barata LT, Ying S, Grant JA, Humbert M, Barkans J, Meng Q et al. Allergen-induced recruitment of FcεRI+ eosinophils in human atopic skin. European Journal of Immunology. 1997 Mar;27(5):1236-1241.
Barata, Luis T. ; Ying, Sun ; Grant, J. Andrew ; Humbert, Marc ; Barkans, Julia ; Meng, Qui ; Durham, Stephen R. ; Kay, A. Barry. / Allergen-induced recruitment of FcεRI+ eosinophils in human atopic skin. In: European Journal of Immunology. 1997 ; Vol. 27, No. 5. pp. 1236-1241.
@article{d7079ad68f6a49c385bfe3da7d0a4d82,
title = "Allergen-induced recruitment of FcεRI+ eosinophils in human atopic skin",
abstract = "We have attempted to identify FcεRI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30{\%} expressed mRNA for the α, β, and γ chains of FcεRI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-a chain antibody, the FcεRIα protein also co-localized to approximately 50-80{\%} of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of FcεRI+ (997+)/EG2+ eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express FcεRI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo.",
keywords = "Allergy, Eosinophils, Fc receptors, Skin",
author = "Barata, {Luis T.} and Sun Ying and Grant, {J. Andrew} and Marc Humbert and Julia Barkans and Qui Meng and Durham, {Stephen R.} and Kay, {A. Barry}",
year = "1997",
month = "3",
language = "English (US)",
volume = "27",
pages = "1236--1241",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "5",

}

TY - JOUR

T1 - Allergen-induced recruitment of FcεRI+ eosinophils in human atopic skin

AU - Barata, Luis T.

AU - Ying, Sun

AU - Grant, J. Andrew

AU - Humbert, Marc

AU - Barkans, Julia

AU - Meng, Qui

AU - Durham, Stephen R.

AU - Kay, A. Barry

PY - 1997/3

Y1 - 1997/3

N2 - We have attempted to identify FcεRI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30% expressed mRNA for the α, β, and γ chains of FcεRI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-a chain antibody, the FcεRIα protein also co-localized to approximately 50-80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of FcεRI+ (997+)/EG2+ eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express FcεRI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo.

AB - We have attempted to identify FcεRI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30% expressed mRNA for the α, β, and γ chains of FcεRI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-a chain antibody, the FcεRIα protein also co-localized to approximately 50-80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of FcεRI+ (997+)/EG2+ eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express FcεRI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo.

KW - Allergy

KW - Eosinophils

KW - Fc receptors

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=0030959608&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030959608&partnerID=8YFLogxK

M3 - Article

C2 - 9174616

AN - SCOPUS:0030959608

VL - 27

SP - 1236

EP - 1241

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 5

ER -