Allergenicity assessment of osmotin, a pathogenesis-related protein, used for transgenic crops

Prerna Sharma, Abinav Kumar Singh, Bhanu Pratap Singh, Shailendra Nath Gaur, Naveen Arora

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Genetic engineering can enhance abiotic stress tolerance of plants, thereby increasing productivity. The present study investigates allergenicity of osmotin protein used for developing transgenic crops. Bioinformatic analysis of osmotin was performed using SDAP and Farrp allergen databases. Osmotin was cloned in pET22b+ vector, purified to homogeneity, and analyzed for digestibility, heat stability, and IgE binding using atopic patients' sera. Osmotin showed 40-92% and 48-75% homology with allergens in SDAP and Farrp databases, respectively. These cross-reactive allergens were from apple, tomato, peach, capsicum, kiwi fruit, and cypress. Osmotin was resistant to pepsin digestion and heat treatment at 90 °C for 1 h. Osmotin protein showed dose-dependent inhibition with pooled patients' sera. It showed significant IgE binding with 22 of 117 patients' sera who were sensitized to tomato and apple, thus indicating cross-reactivity among tomato, apple, and osmotin allergens. In conclusion, osmotin was identified as a potential allergen and showed cross-reactivity with tomato and apple allergens.

Original languageEnglish (US)
Pages (from-to)9990-9995
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number18
DOIs
StatePublished - Sep 28 2011
Externally publishedYes

Fingerprint

allergenicity
pathogenesis-related proteins
allergens
Allergens
Crops
Malus
genetically modified organisms
Lycopersicon esculentum
crops
apples
tomatoes
Proteins
cross reaction
Immunoglobulin E
Hot Temperature
Serum
Cupressus
Databases
Genetic engineering
Capsicum

Keywords

  • allergenicity
  • cross-reactivity
  • food allergy
  • Genetic engineering
  • genetically modified food
  • Nicotiana tabacum
  • osmotin
  • pathogenesis related protein
  • transgenic crops

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Allergenicity assessment of osmotin, a pathogenesis-related protein, used for transgenic crops. / Sharma, Prerna; Singh, Abinav Kumar; Singh, Bhanu Pratap; Gaur, Shailendra Nath; Arora, Naveen.

In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 18, 28.09.2011, p. 9990-9995.

Research output: Contribution to journalArticle

Sharma, Prerna ; Singh, Abinav Kumar ; Singh, Bhanu Pratap ; Gaur, Shailendra Nath ; Arora, Naveen. / Allergenicity assessment of osmotin, a pathogenesis-related protein, used for transgenic crops. In: Journal of Agricultural and Food Chemistry. 2011 ; Vol. 59, No. 18. pp. 9990-9995.
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abstract = "Genetic engineering can enhance abiotic stress tolerance of plants, thereby increasing productivity. The present study investigates allergenicity of osmotin protein used for developing transgenic crops. Bioinformatic analysis of osmotin was performed using SDAP and Farrp allergen databases. Osmotin was cloned in pET22b+ vector, purified to homogeneity, and analyzed for digestibility, heat stability, and IgE binding using atopic patients' sera. Osmotin showed 40-92{\%} and 48-75{\%} homology with allergens in SDAP and Farrp databases, respectively. These cross-reactive allergens were from apple, tomato, peach, capsicum, kiwi fruit, and cypress. Osmotin was resistant to pepsin digestion and heat treatment at 90 °C for 1 h. Osmotin protein showed dose-dependent inhibition with pooled patients' sera. It showed significant IgE binding with 22 of 117 patients' sera who were sensitized to tomato and apple, thus indicating cross-reactivity among tomato, apple, and osmotin allergens. In conclusion, osmotin was identified as a potential allergen and showed cross-reactivity with tomato and apple allergens.",
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