Allosteric inhibitors of Coxsackie virus A24 RNA polymerase

Catherine H. Schein, Diane Rowold, Kyung Choi

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Coxsackie virus A24 (CVA24), a causative agent of acute hemorrhagic conjunctivitis, is a prototype of enterovirus (EV) species C. The RNA polymerase (3Dpol) of CVA24 can uridylylate the viral peptide linked to the genome (VPg) from distantly related EV and is thus, a good model for studying this reaction. Once UMP is bound, VPgpU primes RNA elongation. Structural and mutation data have identified a conserved binding surface for VPg on the RNA polymerase (3Dpol), located about 20Å from the active site. Here, computational docking of over 60,000 small compounds was used to select those with the lowest (best) specific binding energies (BE) for this allosteric site. Compounds with varying structures and low BE were assayed for their effect on formation of VPgU by CVA24-3Dpol. Two compounds with the lowest specific BE for the site inhibited both uridylylation and formation of VPgpolyU at 10-20μM. These small molecules can be used to probe the role of this allosteric site in polymerase function, and may be the basis for novel antiviral compounds.

    Original languageEnglish (US)
    JournalBioorganic and Medicinal Chemistry
    DOIs
    StateAccepted/In press - Oct 9 2015

    Fingerprint

    Enterovirus
    DNA-Directed RNA Polymerases
    Binding energy
    Viruses
    Allosteric Site
    Uridine Monophosphate
    RNA Polymerase III
    Acute Hemorrhagic Conjunctivitis
    Antiviral Agents
    Elongation
    Genes
    RNA
    Peptides
    Molecules
    Catalytic Domain
    Binding Sites
    Genome
    Mutation

    Keywords

    • +-Strand RNA virus
    • Antiviral compounds
    • Coxsackie virus
    • Docking compound libraries
    • Enterovirus RNA polymerase
    • Poliovirus
    • Protein-primed RNA synthesis
    • Uridylylation mechanism

    ASJC Scopus subject areas

    • Biochemistry
    • Clinical Biochemistry
    • Molecular Biology
    • Molecular Medicine
    • Organic Chemistry
    • Drug Discovery
    • Pharmaceutical Science

    Cite this

    Allosteric inhibitors of Coxsackie virus A24 RNA polymerase. / Schein, Catherine H.; Rowold, Diane; Choi, Kyung.

    In: Bioorganic and Medicinal Chemistry, 09.10.2015.

    Research output: Contribution to journalArticle

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