In the present study we describe changes in aorta at the protein level associated with allylamine (AA) and β-aminopropionitrile (βAPN) induced vascular toxicity in a rat model. This model represents a remarkable synergistic, necrotizing toxic effect of these combined toxins, and our rationale was to examine protein expression in order to shed light on the mechanisms underlying this synergism. Rats were given AA (100 mg kg body weight day) and βAPN (1 g kg body weight day) by gavage for 10 d; this protocol has been shown to result in smooth-muscle necrosis, but no visible connective tissue changes. Soluble and insoluble fractions from AA + βAPN- or from βAPN-treated aorta showed enhanced expression of three high-molecular-weight protein bands (ranges between approximately 120 and 95 kD). The time course of induction of proteins showed the appearance of AA + βAPN-induced specific proteins at d 3 of AA + βAPN treatment. Partial purification and characterization suggested that AA + βAPN specific proteins are likely to be collagen proteins (type I). Thus, the data presented in this article help in understanding the vascular toxicity induced by AA + βAPN or by βAPN, in that we have described an altered phenotypic expression of collagenous proteins indicative of selective medial vascular toxicity.
|Original language||English (US)|
|Number of pages||16|
|Journal||Journal of Toxicology and Environmental Health - Part A|
|State||Published - Jan 1998|
ASJC Scopus subject areas
- Health, Toxicology and Mutagenesis