Allylamine cardiovascular toxicity: VI. subcellular distribution in rat aortas

Robert M. Hysmith, Paul J. Boor

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Abstract

The cardiovascular toxin allylamine (3-aminopropene) has been shown to concentrate in elastic and muscular tissues. In this study the 14C-moiety of [14C] allylamine was traced in aortas of adult Sprague-Dawley rats after intravenously injecting 30 μ;Ci of [14C] allylamine (spec. act. = 0.4 mCi/mM). At 5, 10, 15 and 20 min after injection 33.3-29.8% of the 14C-moiety was sequestered in aortas; at 30 min 16.8% was still present. Subcellular fractionation of the postnuclear supernatant by isopycinic centrifugation in sucrose demonstratated that 5 min after administration of [14C] allylamine, the 14C-moiety displayed a modal density peak of 1.20 g/ml. Similar activities were observed up to 30 min exposure. This modal density was similar to the distribution pattern of mitochondria based on analysis of malate dehydrogenase activities. As early as 20 min post-exposure, mitochondrial malate dehydrogenase activities of aortic mitochondria decreased, while cytosolic malate dehydrogenase activities increased, suggesting mitochondrial membrane perturbation. We suggest that the subcellular site for allylamine injury to the aorta is the mitochondrion.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalToxicology
Volume35
Issue number3
DOIs
StatePublished - Jun 14 1985

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Keywords

  • Allylamine
  • Aorta
  • Subcellular distribution
  • Subcellular fractionation

ASJC Scopus subject areas

  • Toxicology

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